Stress-endureing cells from oral tissues for bone engineering

将人牙周膜细胞暴露于严重低氧(O2&lt；0.1%)培养条件下，分离出可用于骨工程的耐应激细胞。在此条件下存活12h的细胞显示出高表达的多能干细胞标志和间充质干细胞标志，并且在体外也表现出最高的多能性。特别是，这些存活的细胞在复氧后可以在成骨介质中形成丰富的钙化结节。此外，这些应激耐受细胞和纤维蛋白凝胶的复合体在体内显示了显著的成骨诱导作用。

Human periodontal ligament cells were exposed to severe hypoxic (O2<0.1%) condition in culture to isolate the stress-enduring cells for bone engineering. Cells survived under this condition for 12 hours showed the high expression of pluripotent stem cell markers as well as mesenchymal stem cell markers, and also displayed the highest pluripotency in vitro. In particular, these surviving cells after re-oxygenation could form abundant calcified-nodules in osteogenic medium. Furthermore, a complex with these stress-enduring cells and fibrin-gel showed the remarkable osteoinducibility in vivo.