The transport mechanism of the peptide transporter by the X-ray structual anaysis.

我们试图将PepTSo和抗体的复合物共结晶。此外，我们还试图生成PEPT同源物的抗体，其中一个同源物来自嗜热链球菌（PepTSt），两个同源物来自拟南芥（PTR1和PTR2）。当我们产生针对PepTSo的抗体时，我们成功地产生了一些针对三种同源物的抗体。这些抗体识别PEPT同源物的构象表位。经气相扩散法初步筛选，发现PepTSt与该抗体的配合物呈一致结晶。晶体在3.5埃左右衍射。我们现在正在进行功能和结构分析。

We were trying to co-crystallize the complex of the PepTSo and antibody. Furthermore we tried to generate antibodies against PEPT homologues, one homologue was from Streptococcus thermophilus (PepTSt) and two were from Arabidopsis thaliana (PTR1 and PTR2). As we generated antibodies against PepTSo, we were successful to produce some antibodies against three homologues. These antibodies recognized conformational epitope of the PEPT homologues. On initial screening by vapor diffusion method, the complex of the PepTSt and this antidody conformed crystals. The crystals were diffracted at about 3.5 angstrom. We are now proceeding the functional and structural analyses.