Dynamic status and senescence of hepatic stem cells in injured rat liver

损伤大鼠肝脏中肝干细胞的动态状态和衰老

• 批准号: 17591344
• 负责人: OGASAWARA Satoshi
• 金额: $ 2.24万
• 依托单位: Iwate Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591344/
• 关键词: anaplastic thyroid cancer; cell cycle; Chk1; UCN-01; apoptosis; papillary thyroid cancer; ATP assay; 甲状腺未分化癌

Anaplastic thyroid cancer (ATC) has an even worse prognosis, and they have not cured by the surgery with a very low chance of surviving from the disease. Tb develop a new strategy for the treatment of ATC, we examine Chk1 inhibitor (UCN-01) in ATC cell lines.We determined expression and/or phosphorylation status of ART, ATM, and Chk1 in ATC cell lines using Western blotting. Proliferation activities were measured by ATP assays.Marked repression and apoptosis was observed in ATC cells after the treatment with UCN-01, in dose- and time dependent manner. Furthermore, we confirmed down-regulation of ART, ATM, and Chk1 phosphorylation. MiR-138 was significantly down-regulated in ATC cell lines in comparison with papillary thyroid cancer cell lines. Its loss of miR-138 was closely associated with Chk1 status in ATC cell lines.Our preclinical trials suggested that UCN-01 was useful for the management of ATC patients, and the miR-138 expression might regulate the Chk1 phosphorylation status.

甲状腺间变性癌（ATC）的预后更差，手术无法治愈，存活几率极低。为了开发治疗ATC的新策略，我们在ATC细胞系中检测Chk1抑制剂（UCN-01）。我们使用Western blotting检测ATC细胞系中ART、ATM和Chk1的表达和/或磷酸化状态。通过ATP测定细胞增殖活性。UCN-01对ATC细胞有明显的抑制和凋亡作用，并呈剂量和时间依赖性。此外，我们证实了ART、ATM和Chk1磷酸化的下调。与甲状腺乳头状癌细胞系相比，ATC细胞系中MiR-138明显下调。在ATC细胞系中，miR-138的缺失与Chk1状态密切相关。我们的临床前试验表明，UCN-01对ATC患者的管理是有用的，miR-138的表达可能调节Chk1磷酸化状态。

项目成果

Infrequent mutation of the human envoplakin gene is closely ranked to the tylosis oesophageal cancer locus in sporadic oesophageal squamous cell carcinomas

人类 envoplakin 基因的罕见突变与散发性食管鳞状细胞癌中的典型食管癌基因座紧密相关

• 发表时间: 2005
• 期刊: Oncol Rep 13
• 作者: Iwaya T;Maesawa C;Kimura T;et al.
• 通讯作者: et al.

Expression of human telomerase reverse transcriptase splice variants is well correlated with low telomerase activity in osteosarcoma cell lines

人端粒酶逆转录酶剪接变体的表达与骨肉瘤细胞系中的低端粒酶活性密切相关

• 发表时间: 2005
• 期刊: Int J Oncol. Apr 26(4)
• 作者: Fujiwara-Aikita H;Maesawa C;Honda T;Kobayashi S;Masuda T.
• 通讯作者: Masuda T.

True carcinosarcoma of the esophagus

• DOI: 10.1111/j.1442-2050.2006.00538.x
• 发表时间: 2006-01-01
• 期刊: DISEASES OF THE ESOPHAGUS
• 影响因子: 2.6
• 作者: Iwaya, T;Maesawa, C;Masuda, T
• 通讯作者: Masuda, T

Interferon-gamma down-regulates expression of tumor necrosis factor-alpha converting enzyme/a disintegrin and metallooroteinose 17 in activated hepatic stellate cells of rats.

干扰素-γ 下调大鼠活化肝星状细胞中肿瘤坏死因子-α 转换酶/解整合素和金属蛋白糖 17 的表达。

• 发表时间: 2006
• 期刊: Int J Mol Med 17
• 作者: Fujita T;Maesawa C;Oikawa K;et al.
• 通讯作者: et al.

Mutations of the epidermal growth factor receptor gene in gastrointestinal tract tumor cell lines.

• DOI: 10.3892/or.15.5.1205
• 发表时间: 2006-05
• 期刊: Oncology reports
• 影响因子: 4.2
• 作者: Toshimoto Kimura;C. Maesawa;K. Ikeda;G. Wakabayashi;T. Masuda