Dynamic status and senescence of hepatic stem cells in injured rat liver

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Anaplastic thyroid cancer (ATC) has an even worse prognosis, and they have not cured by the surgery with a very low chance of surviving from the disease. Tb develop a new strategy for the treatment of ATC, we examine Chk1 inhibitor (UCN-01) in ATC cell lines.We determined expression and/or phosphorylation status of ART, ATM, and Chk1 in ATC cell lines using Western blotting. Proliferation activities were measured by ATP assays.Marked repression and apoptosis was observed in ATC cells after the treatment with UCN-01, in dose- and time dependent manner. Furthermore, we confirmed down-regulation of ART, ATM, and Chk1 phosphorylation. MiR-138 was significantly down-regulated in ATC cell lines in comparison with papillary thyroid cancer cell lines. Its loss of miR-138 was closely associated with Chk1 status in ATC cell lines.Our preclinical trials suggested that UCN-01 was useful for the management of ATC patients, and the miR-138 expression might regulate the Chk1 phosphorylation status.