Multiscale motion analysis of membrane proteins at neuromuscular junction

在本项目中，我们致力于开发基于衍射x射线跟踪法（DXT）在多时间尺度上跟踪膜蛋白内部运动和平移运动的实验技术。在DXT中，单个蛋白质的内部运动是通过固定在底物表面的目标蛋白质上标记的纳米晶体的劳厄点轨迹来监测的。我们改进了金纳米晶体的质量，并优化了实验系统，以监测配体诱导的膜蛋白，乙酰胆碱受体的运动，在几个10微秒的尺度上。此外，我们研究了量子点作为DXT探针，可以适用于跟踪膜蛋白的内部运动（DXT）和平移运动（荧光显微镜）。

In this project, we engaged in developing the experimental technique to track internal motion and translational motion of membrane proteins at multi-time scale based on diffracted X-ray tracking method (DXT). In DXT, the internal motion of an individual single protein is monitored through the trajectory of the Laue spot from the nanocrystal labeled on the objective protein immobilized on the substrate surface. We improved a quality of the gold nanocrystal and optimized the experimental system to monitor ligand-induced motion of membrane protein, acetylcholine receptors, at several 10 micro seconds scale. Moreover, we examined a quantum dot as a DXT probe, that could be suited to track internal motion (DXT) and translational motion (fluorescence microscope) of membrane proteins.