small RNA mediated DNA methylation

逆转录转座子占据了大约40%的基因组DNA，众所周知，DNA甲基化介导的逆转录转座子抑制对哺乳动物至关重要。在男性生殖细胞分化过程中，DNA甲基化被全局清除，随后以特定位点的方式获得DNA甲基化，包括反转录转座子。由于DNA甲基化导致基因抑制，因此DNA甲基化的位点特异性至关重要，然而，如何确定DNA甲基化的位点特异性仍然是难以捉摸的。通过遗传分析，Mili和Miwi2被确定为决定DNA甲基化位点特异性的重要基因，然而，精确的分子机制在很大程度上是未知的。在这个项目中，我报道了MILI和MIWI2如何促进逆转录转座子DNA甲基化的位点特异性

Retrotransposon occupies approximately 40% of genome DNA, and it is widely known that DNA methylation mediated retrotransposon repressions are critical for mammal. During male germ cell differentiation, DNA methylation is globally erased, followed by obtaining DNA methylation at site specific manner, including Retrotransposon. Since DNA methylation causes gene repression, site specificity of DNA methylation is critical, however, it remains elusive how site specificity of DNA methylation is determined. By genetic analysis, Mili and Miwi2 were identified as important genes for to determine site specificity for DNA methylation, however, the precise molecular mechanisms are largely unknown. In this project, I have reported how MILI and MIWI2 contribute to site specificity of DNA methylation for retrotransposon