Accumulation of reactive oxygen species in soybean under waterlogging

研究了大豆根中超氧阴离子(O2-)和过氧化氢(H2O2)的积累。生长1周的大豆受涝影响1周。解除胁迫1d后，取自来根进行O2-和H2O2染色。在自来根的中部观察到O2-染色。根的皮层细胞质膜上检测到H_2O_2的积累。因此，淹水诱导了自来根皮层细胞中O2-和O2产生的过氧化氢的过量积累。我们还研究了大豆播种98天后的生长和产量，大豆在生长早期经历了淹水。冠根生长、籽粒和根瘤重、上部叶片叶绿素含量和光合作用速率均低于对照。这些结果表明，生长前期的渍水导致生长减慢，后期叶片提早黄化。

We investigated the accumulation of superoxide (O2-) and hydrogen peroxide (H2O2) of soybean roots. Soybeans grown for 1 week were affected by waterlogging for 1 week. One day after removing the stress, tap roots were used for the staining of O2- and H2O2. O2- staining was observed in the middle region of a tap root. The accumulation of H2O2 was detected on plasma membrane in a cortical cell of a tap root. Thus, waterlogging induces the excess accumulation of O2- and O2- derived H2O2 in a cortical cell of a tap root.We also investigated the growth and yields 98 days after sowing of soybean, which was subjected by waterlogging in an early growth stage. The shoot and root growth, grain and nodule weight, chlorophyll contents and photosynthesis rate of fully uppermost leaves decreased compared with those of the control. These results suggest that waterlogging in the early growth stage induces the decrease in growth and the earlier etiolation of leaves in the late growth stage.