Do LAM cells express VEGF-D through the activation of mTORC1/ HIF-1a/ VEGF pathway?

在本研究中，我们通过免疫组织化学和免疫印迹分析来阐明LAM细胞是否通过激活哺乳动物靶标雷帕霉素复合体1(MTORC1)/缺氧诱导因子-1a(HIF-1a)/血管内皮生长因子(VEGF)途径来表达血管内皮生长因子-D。所有患者的LAM细胞均表达p-70S6K、p-S6、p-4E-BP1和VEGF-D。另一方面，在LAM细胞中未检测到HIF-1a的核表达。Western印迹分析证实LAM细胞不表达HIF-1a。我们的结果提示LAM细胞通过mTORC1/HIF-1a/VEGF非依赖性途径表达VEGF-D。

In the present study, we performed immunohistochemistry and western blot analysis to elucidate whether LAM cells express Vascular endothelial growth factor (VEGF)-D through the activation of mammalian target of rapamycin complex 1(mTORC1)/ Hypoxia Inducible Factor-1a (HIF-1a)/ VEGF pathway. LAM cells were immunopositive for p-70S6K, p-S6, p-4E-BP1, and VEGF-D in all patients examined. On the other hand, no nuclear expression of HIF-1a was detected in any LAM cells. Western blot analysis confirmed no expression of HIF-1a in LAM cells. Our results suggest that LAM cells expressed VEGF-D though mTORC1/HIF-1a/VEGF-independent pathway.