Functional analysis of human herpesvirus 6 (HHV-6) glycoprotein Q1 (gQ1)

人类疱疹病毒6型由于其临床分离株性质的巨大差异，已被重新归类为两种病毒。在这里，我们重点关注HHV-6A和-6B的gQ1基因，这两个基因已被报道对这些病毒的入侵起重要作用。我们发现，在感染环境中，HHV-6B gQ1不能替代HHV-6A gQ1功能。此外，我们还发现HHV-6A gQ1的N端含有与CD46、HHV-6A进入受体结合的部位。此外，我们还鉴定了HHV-6B gQ1功能所需的氨基酸残基。

Human herpesvirus 6 has been reclassified as two virus species because of the huge different character of its clinical isolates. Here we focused gQ1 genes of HHV-6A and -6B, which have been reported to be important for these viruses' entry. We found that HHV-6B gQ1 could not substitute HHV-6A gQ1 function in the infection context. Furthermore, we found that the N terminal of HHV-6A gQ1 contains the site for the binding of CD46, HHV-6A entry receptor. Furthermore we also identified an amino acid residue required for HHV-6B gQ1 function.