Coordinated epigenetic regulation of cell type-specific genes and chronic disease development in cardiomyocytes.

在这项研究中，我制作了几个细胞类型特异的DNA甲基化表观基因组数据，从几个发育阶段分离的心肌细胞群体中，推动了对成熟细胞表观基因组的理解。我将心肌细胞表观基因组与胚胎干细胞和肝脏的表观基因组进行了比较，也与心力衰竭的心肌细胞进行了比较。结果，在其基因体区DNA甲基化发生显著变化的少数基因亚群被包括在细胞类型特定的基因特征中。这些基因体区在成熟细胞中积累了转录机制和组蛋白乙酰化酶，并瞬时积累了甲基胞嘧啶去甲基化的衍生物，这意味着活跃的去甲基化过程。我们的研究表明，活跃的DNA去甲基化过程参与了基因体区域，并表明基因体DNA低甲基化可能稳定了细胞类型特异性基因的转录。

In this study, I made several cell type-specific DNA methylation epigenome data of isolated cardiomyocyte populations from several developmental stages and pushed forward understanding of the epigenome in matured cells. I compared the cardiomyocyte epigenome with those of embryonic stem cells and liver, and also with the heart failure cardiac muscle cells. As the result, small subpopulation of genes with remarkable DNA methylation changes in their gene body regions were included in the cell type-specific gene trait. These gene body regions accumulated the transcription machinery and histone acetylation enzyme in the matured cells, and transiently accumulate the derivatives of methyl cytosine demethylation, which implicates the active demethylation process. Our study showed the involvement of the active DNA demethylation process in gene body regions and indicates the possibility that the gene body DNA hypomethylationmay stabilize the cell type-specific gene transcription.