Microbial analysis of subglacial samples drilling at Dome Fuji, Antarctica

南极洲的冰芯可以保存古代大气中的微生物，这些微生物被困在冰盖的冰雪中。冰下环境被认为是古老的生态系统。我们用分子DNA技术检测了基岩附近冰芯样品中的微生物。利用专门为生物分析设计的融化装置对冰芯样品内部进行了提取。从冰样品中提取小的DNA片段，并使用Illumina Hiseq和Miseq测序仪进行测序。我们对宏基因组DNA进行了分类和功能分类，并试图重建生物体。我们的数据支持微生物存在于这个冰下环境。

The ice cores from Antarctica could preserve microorganisms in the ancient atmosphere trapped in the snow and ice from the ice sheet. Subglacial environment are thought to be contained ancient ecosystems. We examine microorganisms in the ice core samples near the bedrock by molecular DNA techniques. We extracted the inner part of ice core samples using the melting device special designed for biological analysis. Small DNA fragments were extracted from ice samples and sequenced using Illumina Hiseq and Miseq sequencers. We carried out taxonomic and functional binning of the metagenomic DNA, and attempted to reconstruct the organisms. Our data support that microorganism have existed in this subglacial environment.