Einfluss von Schwermetallen (Zink) auf die Pathogenität von Salmonella enterica pv. Typhimurium

在我的研究中，我将研究铁以外的金属在细菌毒力中的作用。有许多病原微生物需要金属才能致病，包括结核分枝杆菌、脑膜炎奈瑟氏菌和肠道沙门氏菌。我将首先使用沙门氏菌作为模式生物，因为它很容易进行基因操作。肠道沙门氏菌致病变种鼠伤寒具有广泛的宿主范围，并且其例如导致人类胃肠炎。在小鼠模型S.肠病毒鼠伤寒可以作为由S.肠病毒人类伤寒铁、锰等不同微量元素对S.肠病毒鼠伤寒沙门氏菌部分研究良好。初步了解锌在沙门氏菌毒力中的作用。肠病毒鼠伤寒沙门氏菌，第一个目标是：i）表征锌转运蛋白在感染和存活中的作用，ii）开始鉴定和表征参与毒力的Zn 2+依赖性酶，和iii）表征ZupT（确定膜拓扑结构和表征ZupT介导的金属转运）。

In my research I will investigate the role of metals other than iron in bacterial virulence. There are many pathogenic microorganisms that require metals for virulence including Mycobacterium tuberculosis, Neisseria meningitis and Salmonella enterica. I will first employ Salmonella as a model organism because it is easily genetically manipulated. Salmonella enterica pv. Typhimurium has a broad host range and it is for example responsible for human gastroenteritis. In the mouse model S. enterica pv. Typhimurium can serve as a model for typhoid fever caused by S. enterica pv. Typhi in humans. The role of different trace elements, such as iron and manganese, on virulence of S. enterica pv. Typhimurium are partially well investigated. Starting to understand the role of zinc in virulence of S. enterica pv. Typhimurium, the frist goals are: i) characterization the role of zinc transporters in infection and survival, ii) starting identification and characterization of Zn2+-dependent enzymes involved in virulence, and iii) characterization of ZupT (determining membrane topology and characterization of ZupT mediated metal transport).