ニホンザルとオランウータンにおける内分泌動態に影響を与える要因について

我开发了一种新的酶免疫测定法（EIA），以检测日本猕猴和猩猩在过去一年中收集的粪便中的硫酸脱氢表雄酮（DHEAS）水平。我通过确认一只雄性和一只雌性日本猕猴在DHEA激发后DHEAS水平增加来验证该测定。在其他猴子中使用促肾上腺皮质激素（ACTH）和人绒毛膜促性腺激素（hGC）的其他挑战表明，日本猕猴中ACTH和hCG的肌内注射不会刺激肾上腺分泌DHEAS。这项研究发表在《国际灵长类动物学杂志》上。我还发表了从去年的结果在journla灵长类动物，有关的压力减轻效果的温泉的压力水平的日本猕猴。该项目的另一部分包括验证的EIA测量DHEAS在猩猩粪便。我调查了激素变化（DHEAS和糖皮质激素）水平：（a）应激反应，（B）生殖状态，（c）圈养和野生猩猩的年龄。结果表明，DHEAS上升响应GC水平的增加。我还发现怀孕的女性DHEAS水平最高，早期哺乳期女性的fGC水平最高。最后，我发现DHEAS在婴儿中低于成人和青少年，青春期前DHEAS水平增加。这种现象被称为肾上腺初显，它存在于人类、黑猩猩和倭黑猩猩中，但不存在于猕猴和低等灵长类动物中。

I developed a new Enzyme Immunoassay (EIA) to detect dehydroepiandrosterone-sulfate (DHEAS) levels in feces of Japanese macaques and orangutans collected in the previous year. I validated the assay by confirming that DHEAS levels increased after DHEA challenge in one male and one female Japanese macaques. Additional challenges using Adrenocorticotrophic hormone (ACTH) and human chorionic gonadotropin(hGC) in other monkeys revealed that the intra-muscular injection of ACTH and hCG did not stimulate the adrenal to secrete DHEAS in Japanese macaques. The study has been published at the International Journal of Primatology. I also published the results from the previous year in the journla Primates, related to the stress-reducing effect of hot springs in stress levels of Japanese macaques.Another part of this project included the validation of the EIA for measurement of DHEAS in feces of orangutans. I investigated hormonal changes (DHEAS and glucocorticoids) levels with: (a) stress response, (b) reproductive state, and (c) age in orangutans from captivity and from the wild. The results suggest that DHEAS rises in response to increase in GC levels. I also found that pregnant females had the highest levels of DHEAS, and that early lactating females had the highest levels of fGC. Finally, I found that DHEAS is lower in infants than in adults and adolescents, with a pre-pubertal increase on DHEAS levels. This phenomenon is called adrenarche, and it is present in humans, chimpanzees, and bonobos, but not in macaques and lower primates.