Tuning the master clock of the brain: Endocannabinoid modulation of the olivo-cerebellar system and animal behavior

在2019财年，JSPS支持的项目进入了最后阶段。综上所述，JSPS的资助使我们能够开发和建立一个范式转换的实验框架，用于极高分辨率地跟踪自由行为的老鼠的全身运动，这是以前从未尝试过的。现有的方法依赖于永久植入的皮肤标记和一组专用高速摄像机，这些摄像机允许前所未有的多功能性实时跟踪动物的运动。作为该方法的概念验证，我们用它来研究内源性大麻素（eCB）调节中枢神经系统的微妙运动特征，这是医学药理学中非常重要的主题。借助借助JSPS建立的记录系统，我们可以第一次1)描述以前被忽视的低剂量相关运动效应，以及2)揭示eCB调制的差异，行为状态依赖和分子途径依赖效应。第二部分非常令人惊讶，因为它违背了欧洲央行研究界普遍接受的概念。在FY19中，研究者通过会议演讲的方式广泛宣传了所获得的结果。值得注意的是，虽然联委会的经费是她参加会议的基本经费，但也有几次活动是她被东道主邀请作为演讲者，旅费由她承担。这些活动是在日本推广科学的一个很好的场所。

In FY19, the project supported by JSPS reached its final stage. As a summary, the JSPS funding had allowed us to develop and establish a paradigm-shifting experimental framework for extremely high-resolution tracking of full body movement in freely-behaving mice, something that had not been even attempted before. The established method relies on permanently-implanted skin markers and a set of dedicated high-speed cameras that allow unprecedented versatility to tracking animal movement in real time.As a proof-of-concept for this method, we used it to investigate subtle kinematic signatures of endocannabinoid (eCB) modulation of the central nervous system, a topic that is of burning importance in medical pharmacology. With the aid of the recording system established with the aid of JSPS, we could for the first time 1) describe previously-ignored low-dose-related motor effecs as well as 2) uncover the differential, behavior state-dependent and molecular pathway-dependent effects of eCB modulation. The second part was highly surprising as it runs against commonly-accepted concepts within the eCB research community.In FY19, the results obtained were widely publicized by means of conference presentations by the researcher. Notably, while JSPS funding was fundamental for her participation in the conferences, there were several events where she was invited as a speaker by the host, and her travel costs were covered. These events were a great venue to promote Science in Japan.