Regulation of the PQQ-dependent quinoprotein enzyme systems in Pseudomonas aeruginosa

铜绿假单胞菌使用非常有效的酶系统来氧化乙醇和葡萄糖。这两种系统依赖于相同的醌辅因子吡咯喹啉醌。在乙醇氧化系统中，三个基因编码了一个双组分传感器/响应调节器系统和一个额外的响应调节器。他们是按等级制度组织起来的。从调控突变体的研究中，我们知道至少有7个基因参与了乙醇氧化的调控。将确定乙醇氧化系统中缺失的调节因子，并研究它们与已知调节蛋白的相互关系。此外，我们还想知道乙醇和葡萄糖氧化系统的调控网络是如何交织在一起的。这两种体系都需要辅因子PQQ，而藜蛋白乙醇脱氢酶或葡萄糖脱氢酶载脂蛋白仅在各自底物存在时才需要。

P. aeruginosa uses very efficient enzyme systems to oxidize ethanol and glucose. Both systems depend on the same quinone cofactor pyrroloquinoline quinone. With the ethanol oxidation system three genes coding for a two component sensor/response regulator system and an additional response regulator have been identified. They are organized in an hierarchiacal manner. From studies with regulatory mutants, we know that at least 7 genes must be involved in the regulation of ethanol oxidation. The missing regulatory factors of the ethanol oxidation system will be identified and their interrelationship with the known regulatory proteins will be studied. In addition we want to know how the regulatory networks of the ethanol and the glucose oxidation systems are interwoven. Both systems need the cofactor PQQ, while the quinoprotein ethanol dehydrogenase or glucose dehydrogenase apoproteins are needed only in the presence of the respective substrate.