In vitro Germ Cell Differentiation of Cord-Blood-derived Unrestricted Somatic Stem Cells (USSC)

人类脐带血来源的非限制性体细胞干细胞（USSC）可以被重新编程为诱导多能干细胞（iPS）。本项目通过不同的方法从USSC （iP-USSC）中提取和表征iPS细胞。将评估重编程脐带血细胞的分化潜力，并与天然USSC进行比较。该项目的重点包括iP-USSC的中胚层/造血和生殖细胞诱导。此外，将对USSC中胚层祖细胞重编程为完全多能诱导进行评估。

Human cord-blood derived unrestricted somatic stem cells (USSC) can be reprogrammed to induced pluripotent stem (iPS) cells. This project derives and characterizes iPS cells from USSC (iP-USSC) by different means. The differentiation potential of reprogrammed cord blood cells will be assessed in comparison to native USSC. The focus of this project embraces mesodermal / hematopoietic and germ cell induction from iP-USSC. Besides reprogramming to full pluripotency induction of mesodermal progenitors in USSC will be evaluated.