Gingival vaccination strategy to induce both humoral and cellular immunity ideal for elderly vaccination

我们能够制造出一种蛋白质抗原的分子结构，这将在整个研究中使用。更具体地说，来自原始、α、β、γ和δ变体的SARS-CoV-2刺突蛋白被成功地用计算机设计出来。我们能够在同行评审的期刊上发表与SARS-CoV-2计算机设计相关的初步结果。目前，我们同样正在设计与甲型流感H3N2和乙型流感/山形血凝素（HA）蛋白相关的分子结构。此外，我们还决定为分子结构设计添加额外的蛋白质，即：SARS-CoV-2组粒刺突和甲型H5N1 HA蛋白，因为正在进行的大流行主要与组粒变体相关，而正在进行的禽流感流行可能有可能传播给人类。已经设计的蛋白质结构目前正在进行表位筛选，以确定与免疫反应相关的潜在B细胞和t细胞相关构象表位。此外，我们还能够执行并确认黄原胶和抗原对接，涉及一些SARS-CoV-2刺突蛋白。我们目前正在确认与激活B细胞和t细胞免疫应答相关的表位在黄原胶分子对接后是否容易暴露。

We were able to produce the molecular structure of one of the protein antigens that would be used throughout the study. More specifically, the SARS-CoV-2 spike protein from the original, alpha, beta, gamma, and delta variants were successfully designed in silico. We were able to publish our preliminary results related to the in silico design of the SARS-CoV-2 in a peer-reviewed journal. At present, we are likewise designing molecular structures related to the influenza A H3N2 and influenza B/Yamagata hemagglutinin (HA) proteins. Additionally, we also decided to add additional proteins for molecular structure design, namely: SARS-CoV-2 omicron spike and influenza A H5N1 HA proteins since the on-going pandemic is mainly associated with the omicron variant while the on-going bird flu epidemic might have the potential to cross to humans. Protein structures that have already been designed are currently undergoing epitope screening to identify potential B- and T-cell-related conformational epitopes associated with immune response. Moreover, we were also able to perform and confirm xanthan gel and antigen docking involving some of the SARS-CoV-2 spike proteins. We are currently confirming whether epitopes associated with activating B- and T-cell immune responses are readily exposed after xanthan gel molecule docking.