Changes in the biodiversity of algae in soils along land use gradients within the German Biodiversity Exploratories: community structures and functional roles

在生物多样性探索区内，将研究来自不同陆地生境的藻类遗传多样性的变化。由于藻类非常容易受到环境因素的影响，我们假设不同的管理强度反映在藻类群落结构中。将对150个森林和150个草地试验地的扩大样本进行测试，这些试验地涵盖各种土壤类型和不同的管理制度，并在较小规模上对藻类生长的粗木屑（死木）进行测试。将与侧重于土壤和枯木的各个小组密切合作，评估藻类与其他（微）有机体群体的共同发生以及与非生物因素的相关性。通过对土壤的干扰/封闭实验和基于pcr的对枯木和凋落物中微节肢动物的调查，将探索与微节肢动物（藻类的潜在消费者）的功能相关性，即微节肢动物促进藻类扩散的程度。将评估藻类群落的空间变化，以及它们随时间的稳定性。此外，标签编码焦磷酸测序将用于测试以前使用的克隆文库和培养为基础的方法。总的来说，这将提供一个更全面的陆地藻类多样性的生物多样性探索的图片。

Changes in the genetic diversity of algae from various terrestrial habitats within the Biodiversity Exploratories will be studied. Because algae are very susceptible to environmental factors, we hypothesize that different management intensities are reflected in the algal community structures. This will be tested with an expanded sample of 150 forest and 150 grassland experimental plots covering various soil types and different management regimes and, at a smaller scale, coarse woody debris (dead wood) colonized by algae. In close collaboration with various groups focusing on soil and dead wood co-occurence of algae with other (micro-) organismal groups and correlations with abiotic factors will be assessed. With a disturban-ce/exclosure experiment in the soil and PCR-based investigation of microarthropods from dead wood and litter a functional correlation with microarthropods (potential consumers of algae) will be explored, i.e. in as much microarthropods facilitate the dispersal of algae. The spatial variation of the algal communities will be assessed and also how stable they are over time. In addition, Tag-encoded pyrosequencing will be employed to test previously employed clone library- and culture-based methods. Altogether this will provide a more comprehensive picture of the terrestrial algal diversity of the Biodiversity Exploratories.