Trilat. Project: Mango malformation: Epidemiology and simultaneous transcriptome analyses of host and fungal pathogen

芒果(Mangfera Imtica)是以色列重要的果树，有可能成为巴勒斯坦的主要农作物。芒果发展的主要瓶颈之一是由芒果镰刀菌引起的一种毁灭性的疾病-芒果畸形。芒果畸形是一种复杂而神秘的疾病，它引起花序畸形，在世界大部分地区的芒果园蔓延开来，并极大地降低产量，给前者造成重大损失。目前既没有诊断工具，也没有治愈方法，尽管他们非常需要。这一建议的具体目标如下：1.利用SuperSAGE同时鉴定在侵染过程中诱导或抑制的镰刀菌和芒果基因。2.病原来源、感染机制和传播途径的流行病学研究。3.开发一种分子诊断工具，用于在感染的症状前期阶段检测病原体。该项目的预期结果将为流行病学和导致这种目前无法获得的这种独特真菌疾病的感染过程的分子基础提供新的见解。应用新的SuperSage技术，我们将深入了解体内两个密切相互作用的生物的转录组，以及控制芒果花序形成的转录组。

Mango (Mangifera imtica) is an important fruit tree in Israel and has the potential of becoming a major crop in Palestine. One of the major bottlenecks for the development of this crop is a devastating disease - mango malformation - caused i by the fungus Fusarium mangiferae. Mango malformation is a complicated and quite mysterious disease, that causes malformation of the inflorescences, is spread throughout mango orchards in most parts of the world, and dramatically reduces yield, causing major losses to the formers. Neither a diagnostic tool nor a cure is currently available although they are badly needed. The specific goals of this proposal are as follows: 1. Simultaneous identification ofFvsarium and mango genes induced or repressed during the infection process, by SuperSAGE. 2. Epidemiological study of the source of the inoculum, the mechanisms of infection and the ways of dispersal of the pathogen. 3. Development of a molecular diagnostic tool for the detection of the pathogen in pre- symptomatic stages of the infection. The expected results of this project will provide novel insights into both the epidemiology and the molecular basis of the infection process leading to this unique fungal disease that are currently unavailable. Applying the novel SuperSage technique, we will gain in-depth insight into the transcriptomes of two intimately interacting organisms in vivo and the transcriptome controlling the formation of inflorescences in mango.