Effects of prenatal perfluorinated chemicals exposure and genetic vulnerability on glucocorticoids hormone profiles, growth and risk of infectious diseases in next generation

在本研究中，我们试图阐明产前接触全氟化学品对下一代糖皮质激素的影响，在东邦队列的所有参与者（n=514）中，在排除双胞胎和早产儿后，将札幌东邦队列中获得的围产期血清样本中PFOS/PFOA水平的参与者纳入本研究（n=295）。采用液相色谱-串联质谱法（LC-MS-MS）测量了妊娠中期后母体血清样本中的全氟辛烷磺酸和全氟辛酸。在怀孕期间收集基线问卷、出生记录和随访问卷，直至7岁，包括父母人口统计学特征、饮食习惯、社会经济地位、出生大小、儿童生长和传染病等信息（已进行数据清理）。在去年夏天评估了遗传多态性，以确定母亲（分娩时采集的血液样本）和婴儿（脐带血样本）糖皮质激素受体的单核苷酸多态性（SNP）。用EP 1系统（Fluidigm）进行遗传分析。采用LC-MS-MS法对随机抽取的130名受试者的脐带血样本进行了皮质醇和可的松的测定，并进行了数据分析。

In this study, we are trying to clarify the effects of prenatal exposure to perfluorinated chemicals on Glucocorticoid hormones in next generation.Among whole participants in Toho cohort (n=514), participants with available perinatal PFOS/PFOA levels in serum samples obtained in Sapporo Toho cohort were included in this study (n=295) after exclusion of twins and preterm births. Maternal PFOS and PFOA have been measured after the second trimester of pregnancy in serum samples by liquid chromatography-tandem mass spectrometry (LC-MS-MS). Baseline questionnaires, birth records and follow-up questionnaires were collected during pregnancy until 7 years old including information on parental demographic characteristics, dietary habits, socioeconomic status, birth size, children’s growth and infectious diseases (data cleaning has been performed). Genetic polymorphisms were assessed in last summer to determine of maternal (blood samples taken at delivery) and infant (cord blood samples) single nucleotide polymorphisms (SNPs) of glucocorticoid receptors. Genetic analysis were performed by EP1 system (Fluidigm). Cortisol and cortisone levels in cord blood samples of 130 randomly selected subjects have been measured by LC-MS-MS, and I started to do data analysis.