X-ray structure analysis on key enzymes involved in the anaerobic degradation of hydrocarbons

蛋白质结构分析代表了SPP1319计划的生态生理和化学方面之间的重要联系。根据SPP的概念，我们小组从该计划的生物化学家那里获得纯化的蛋白质，以便启动X射线结构分析。在第一个资助期，我们研究了苄基琥珀酰辅酶A硫解酶、苯乙酮羧基酶、苯甲酰辅酶A还原酶、苯甲酰辅酶A环氧酶和甲基辅酶M还原酶。用后三种酶(或酶片段)成功地进行了结构分析。在新的拨款期间，我们计划研究琥珀酰辅酶A(R)苄基琥珀酸辅酶A转移酶、苄基琥珀酸辅酶A硫解酶、丙酮羧基酶、苯乙酮羧基酶、苯甲酰辅酶A还原酶、对甲酚甲基水解酶和甲基硫化物依赖甲基转移酶。

Protein structure analysis represents an essential link between the ecophysiological and the chemical aspects of the SPP1319 program. According to the concept of the SPP our group is supplied with purified proteins from biochemists of the program in order to initiate an X-ray structure analysis. In the first funding period we worked on benzyl-succinyl-CoA thiolase, acetophenone carboxylase, benzoyl-CoA reductase, benzoyl-CoA epoxidase and methyl-coenzyme M reductase. With the latter three enzymes (or enzyme fragments) a successful structure analysis was performed. In the new funding period we plan to work on succinyl-CoA(R) benzylsuccinate CoA transferase, benzyl-succinyl-CoA thiolase, acetone carboxylase, acetophenone carboxylase, benzoyl-CoA reductase, p-cresol methylhydrolase and methyl sulfide dependent methyltransferase.