Center of Isotopologue Profiling (Z-Projekt)

代谢分析的一项关键技术是在受控条件下用稳定同位素（例如13 C）进行标记实验（“代谢通量分析”、“通量组学”）。该技术（改良形式）也可用于研究在准生理（即非常复杂）和医学相关条件下生长的病原微生物的代谢反应。通过许多例子，可以表明同位素分析能够对病原菌及其宿主的碳代谢进行独特的见解。在生物分析平台“同位素体分析中心”（SPP-1316中的Z项目）的框架内，该技术可在任何要求时提供（15个正在进行的项目+ 3个SPP 1316小组计划的项目; 3个正在进行的项目+ 3个SPP以外的请求项目）。在方案的第二个阶段，最高人民检察院的所有成员都应能够使用既定的方法。已经开始和正在进行的合作将继续和扩大。只要有必要，应与最高人民检察院的新团体建立合作关系。应优化和利用同位素谱分析的新工具，以详细分析宿主（即培养中的宿主细胞，但也包括动物和植物）与其病原体之间的代谢相互作用。这还将包括15 N或15 N/13 C标记代谢物的分析，以探索N代谢。为了管理预期的工作量（包括同事和学生的培训），我们要求DFG授予整个优先计划的核心重要性的创新平台。

A key technology for the analysis of metabolism is based on labelling experiments with stable isotopes (e.g. 13C) under controlled conditions (“metabolic flux analysis”, “fluxomics”). The technique (in modified form) can also be used for the study of metabolic reactions in pathogenic microorganisms grown under quasi-physiological (i.e. very complex) and medicinally relevant conditions. With many examples, it could be shown that isotopologue profiling enables unique insights into the carbon metabolism of pathogenic bacteria and their hosts. Within the framework of the bio-analytical platform “Center of Isotopologue profiling” (Z-project in the SPP-1316), the technology was/is made available whenever requested (15 ongoing projects + 3 planned projects with groups of the SPP 1316; 3 ongoing projects + 3 requested projects from outside the SPP). The established methods shall be kept accessible for all members of the SPP during the second period of the program. The already started and ongoing co-operations shall be continued and extended. Co-operations with new groups of the SPP shall be established whenever useful. New tools in isotopologue profiling shall be optimized and exploited for the detailed analysis of the metabolic interaction between host (i.e. host cell in culture, but also animals and plants) and their pathogens. This will also include the analysis of 15N- or 15N/13C-labelled metabolites in order to explore the N-metabolism. To manage the expected work load (including training of co-workers and students), we ask the DFG to grant the innovative platform with central importance of the entire priority program.