Multi-functional probes for global analysis of proteome stress in cells

该项目旨在创建一套多功能化学探针，以识别在压力下细胞中经历解折叠或特定修饰的受损蛋白质。这些探针不仅会产生荧光反应，以反映蛋白质的质量控制能力，但允许相关的蛋白质和它们的网络，以确定在复杂的细胞环境中，这是很难实现目前的方法。预期的结果是提供新的方法，全面了解质量控制机制，应激反应和细胞功能之间的相关性。这应该提供显着的好处，包括有助于神经退行性疾病的分子原因的基础知识。

This project aims to create a suite of multi-functional chemical probes to identify damaged proteins that undergo unfolding or specific modifications in cells under stress. These probes will not only generate fluorescence responses to reflect on protein quality control capacity but allow associated proteins and their networks to be identified in complex cellular environments, which is difficult to achieve by current methods. The expected outcome is to deliver new methodology for a comprehensive understanding of the correlation between quality control machinery, stress responses and cell functions. This should provide significant benefits, including contributing to fundamental knowledge on the molecular causes of neurodegenerative diseases.