Investigation of the repopulation mechanisms in hiPS-derived hepatoblasts through the use of different hiPS-donor

我们使用相同的方案成功地分化了不同的供体-iPS来源的肝母细胞。蛋白(免疫染色、流式细胞仪)和基因表达(qPCR分析)证实了它们的成肝细胞特征。然而，成肝细胞标志的表达存在一定的差异，这意味着这些菌株之间存在株内差异。每一种肝母细胞的白蛋白分泌也通过蛋白检测来确认，成熟为肝细胞的情况通过肝细胞分化来证实。所有细胞都具有在体外增殖的能力，并在整个传代过程中大量扩增。然后将这些细胞移植到慢性肝损伤模型中。

We succeeded in differentiating different donor-iPS-derived hepatoblasts by using the same protocol. Their hepatoblast characters were confirmed by both protein (immunostaining, flow cytometry) and genetic expression (qPCR analysis).Nonetheless, there is some variance in the expression of hepatoblast markers, which implies intra-strain differences between these strains. Each hepatoblast albumin secretion is also confirmed by protein assay and the maturation into hepatocytes is verified by hepatocyte differentiation. All cells possess the ability to proliferate in vitro and largely expanded throughout the passages. These cells are then transplanted into the chronic hepatic injury model.