Structural and functional variations of vertebrate cilia

本研究获得了以下结果：(1)脉络丛上皮细胞(CPECs)独特的9-0纤毛蛋白质组分析鉴定出868个蛋白质；(2)小鼠脉络丛活体成像显示，新生CPEC纤毛能剧烈跳动，运动减弱，在1-2周内丧失活力；(3)超微结构分析显示，新生CPEC中不仅存在9-0纤毛亚型，还存在9-2纤毛亚型和非典型纤毛亚型。(4)DNA芯片分析发现了一组调控每细胞纤毛数量的候选基因。

In the present study, we obtained the following results.(1) Proteomic analysis of unique, multiple 9+0 cilia in choroid plexus epithelial cells(CPECs) identified 868 proteins.(2) Live imaging of mouse choroid plexus revealed that neonatal CPEC cilia could beat vigorously, and the motility waned and was lost within 1-2 weeks.(3) Ultrastructural analysis revealed the presence of not only 9+0 but also 9+2 and atypical ciliary subtypes in neonatal CPEC.(4) A set of candidate genes that would regulate ciliary number per cell was identified based on DNA microarray analysis.