Molecular and cellular biological analysis for the effect of glucocorticoids and their recetors on the function and tissue construction of neuron.

用原位杂交和免疫细胞化学方法研究了GRmRNA和GR免疫反应在成年大鼠脑内的细胞定位。GR mRNA表达定位于神经元胞浆，并见于大鼠脑内不同部位，尤其是在海马区、丘脑、下丘脑、大脑皮层。GR免疫反应阳性细胞的分布模式与GR mRNA阳性细胞的分布模式密切相关，但在海马区(CA3/CA4)的分布与GR受体的分布不同。用免疫细胞化学方法观察了糖皮质激素受体(GR)在不同循环糖皮质激素水平和GR特异性抗体条件下的亚细胞定位。而在…中，免疫反应几乎消失。肾上腺切除(ADX)后2天细胞面积增加。10分钟或15分钟后(Ip)。给ADX大鼠注射地塞米松(200 mg/kg)后，GR免疫反应出现在与细胞器相关的胞浆中。注射地塞米松30min后，GR免疫反应阳性细胞分布在细胞核内，且GRmRNA在垂体前叶细胞和散在分布的垂体后叶细胞胞浆中大量表达，相当于垂体细胞。GR免疫反应阳性细胞定位于垂体前、后叶细胞核。双重免疫组织化学显示，GR和垂体前叶激素在生长激素(GH)和促肾上腺皮质激素(ACTH)的大多数分泌细胞中共存，在约60%的促甲状腺激素(TSH)分泌细胞中共存。对S100蛋白免疫染色的垂体前叶滤泡星状细胞、边缘层细胞和后叶垂体细胞也进行了GR免疫组化染色。提示糖皮质激素可能直接影响某些垂体细胞，调节细胞功能，包括激素的合成和/或分泌。较少

The cellular localization of GRmRNA and GR immunoreactivity in the adult rat brain was studied by in situ hybridization and immunocytochemistry. The expression of GR mRNA was detected in the cytoplasm of neurons and observed in the various regions of the rat brain, especially in the hippocampus, the thalamus, hypothalamus, the cerebral cortex. The distributional pattern of GR-immunoreactive cells was well-correlated with that of GR mRNA-containing cells, but in the hippocampus (CA3/CA4) discrepancy of the distribution of mRNAs and immunoreactivities.Subcellular localization of glucocorticoid receptor (GR) in rat brain was immunocytochemically observed under different conditions of circulating corticosteroids level in conjunction with the specific antibody to GR.In the case of hippocampal pyramidal and granular cells, the GR-immunoreactivity was mainly distributed in the nucleus associated with chromatin in the control (normal) condition. However, the immunoreactivity almost vanished in … More all area of the cells 2 days after adrenalectomy (ADX). After 10 or 15 min of intraperitoneal (i.p.) injection of dexamethasone (DEX ; 200mg/kg) to these ADX rats, the GR-immunoreactivity reappeared in the cytoplasm associated with cell organelles. After 30 min of DEX i.p.injection, the same GR-immunoreactivity was distributed in nucleus as showing the control condition.Moreover, the GRmRNA was abundantly expressed in the cytoplasm of anterior pituitary cells, and of scatteringly posterior pituitary being equivalent to pituicytes. The immunoreactivity for GR was detected in the nuclei of cells in anterior and posterior pituitary. Double immunohistochemistry revealed that co-localization of GR and anterior pituitary hormones were observed in the majority of growth hormone (GH) and adreno-corticotropic hormone (ACTH) producing cells, and in about 60 % of thyroid stimulating hormone (TSH) producing cells. Folliculo-stellate cells, marginal layr cells in the anterior pituitary, and pituicytes in the posterior pituitary, immunostained for S100-protein, were also immunostained with GR.These results suggest that glucocorticoids may directly influence certain pituitary cells and regulate the cell function including the synthesis and/or secretion of hormones. Less