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Interaction of three dimensional network of the feeding regulation neurons and steroid hormones

摄食调节神经元三维网络与类固醇激素的相互作用

基本信息

批准号：
14580772
负责人：
OZAWA Hitoshi
金额：
$ 2.62万
依托单位：
Kyoto Prefectural University of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

It has been reported that there are two orexin (from the Greek word for appetite, orexis) peptides derived from pre-pro-orexin, designated as orexin A and B. Orexin A is a 33-residue peptite with two intramolecular disulfide bonds in the N-terminal region, and orexin B is a linear 28-residue peptide. These peptides, encoded by a single mRNA transcript, has a 46% amino acid sequence identity (Sakurai T et al. 1998). Their effects are mediated by receptors, orexin 1 and 2 receptor. Orexin-A has ha similar affinity for both receptors, whereas orexin-B has a higher affinity for the orexin 2 receptor subtype.It has been also reported that orexin-A and -B mRNA and their cell bodies were mainly detedted in the hypothalamic area, particularly in the lateral hypothalamic area and the perifornical nucleus. Recently, it has been reported that the distribution of immunoreactive fibers overlaps with the luteinizing hormone-releasing hormone(LHRH) neuronal system in the septopreoptic area and the ar … More cuate nucleus median eminence region, suggesting that orexins are involved with the sex hormone secretion. However, the sex difference of the expression of orexin-A and -B has not been elucidated. In the project, we investigated the sex difference of the distribution pattern of the feeding related neurons, particularly orexin neuorns and the effect of estrogen for the expression of orexin peptides in the male, female and ovariectomized (OVX) rats.Orexin neurons are distributed in the perifornical and lateral hypothalamic area, a region classically implicated in the control of feeding behavior, and project their axons within the hypothalamus and throughout the central nervous system to be involved in the control of feeding. In the project, we investigated the sexual difference of orexin neurons using specific orexin-A and orexin-B antibodies. The density of both orexin-A and orexin-B immunopositive neurons in male was preferentially higher than those in female rats. On the other hand, ovariectomy in female rats induced the change to the male pattern. Colocalization of orexin and estrogen receptor was not observed. These results suggested that orexin neurons were indirectly regulated by estrogen through the other estrogen dependent neurons. Less

据报道，有两种食欲素(源于希腊语，意为食欲，orexis)来源于前食欲素原，命名为食欲素A和食欲素B。食欲素A是一种33个残基的多肽，在N-末端有两个分子内二硫键，而食欲素B是一个线性的28个残基的多肽。这些多肽由单个mRNA转录本编码，具有46%的氨基酸序列同源性(Sakurai T等人。1998年)。它们的作用是由食欲素1和2受体介导的。食欲素-A对这两种受体具有相似的亲和力，而食欲素-B对食欲素2受体亚型的亲和力更高。也有报道称，食欲素-A和食欲素-B及其胞体主要在下丘脑区表达，特别是在下丘脑外侧区和穹隆周围核。最近有报道称，免疫反应纤维的分布与黄体生成素释放激素(Lhrh)神经元系统在隔视区和ar…重叠。丘状核正中隆起区较多，提示食欲素与性激素分泌有关。然而，增食欲素-A和-B表达的性别差异尚未阐明。在这个项目中，我们研究了雄性、雌性和去卵巢(OVX)大鼠摄食相关神经元，特别是食欲素神经元的分布模式的性别差异，以及雌激素对食欲素多肽表达的影响。食欲素神经元分布在穹隆周围和下丘脑外侧区，这是一个经典的与摄食行为控制有关的区域，并在下丘脑和整个中枢神经系统内投射轴突，参与摄食控制。在该项目中，我们使用特定的食欲素-A和食欲素-B抗体来研究食欲素神经元的性别差异。雄性大鼠食欲素A和食欲素B免疫阳性神经元密度明显高于雌性大鼠。另一方面，摘除雌性大鼠的卵巢导致了这种向雄性模式的转变。未观察到增食欲素和雌激素受体的共存。这些结果表明，增食欲素神经元是通过其他雌激素依赖神经元间接受雌激素调节的。较少

项目成果

期刊论文数量（48）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Exocytosis sensitivity to growth hormone-releasing hormone in subsets of GH cells in rats under different corticosterone condition. Ultrastructural study using microwave irradiation for fixation

不同皮质酮条件下大鼠 GH 细胞亚群的胞吐作用对生长激素释放激素的敏感性。

DOI：
发表时间：
2004
期刊：
J Endocrinol 183
影响因子：
0
作者：
Ozawa H.;Han F.;Kawata M.
通讯作者：
Kawata M.

ストレスとグルココルチコイド受容体

压力和糖皮质激素受体

DOI：
发表时间：
2002
期刊：
脳の科学 24
影响因子：
0
作者：
小澤一史;謝藏霞;小川弘;西真弓;河田光博
通讯作者：
河田光博

Colocalization of mineralocorticoid receptor and glucocorticoid receptor in the hippocampus and hypothalamus

DOI：
10.1016/j.neures.2004.12.013
发表时间：
2005-04-01
期刊：
NEUROSCIENCE RESEARCH
影响因子：
2.9
作者：
Han, F;Ozawa, H;Kawata, M
通讯作者：
Kawata, M

Steroid efects on secretion from subsets of lactotrophs : role of folliculo-stellate cells and annexin 1.

类固醇对泌乳素亚群分泌的影响：滤泡星状细胞和膜联蛋白 1 的作用。

DOI：
发表时间：
2002
期刊：
Arch Physiol Biochem 110
影响因子：
0
作者：
Morris J.F.;Christian H.C.;Chapman L.P.;Epton M.J.;Buckingham J.C.;Ozawa H.;Nishi M.;Kawata M.
通讯作者：
Kawata M.

Exocytosis sensitivity to growth hormone-releasing hormone in subsets of GH cells in rats under different corticosterone condition. Ultrastructural study using microwave irradiation for fixation and immunocytochemistry.

不同皮质酮条件下大鼠 GH 细胞亚群的胞吐作用对生长激素释放激素的敏感性。