INTRACELLULAR SIGNAL TRANSDUCTION IN DICTYOSTELIUM ANALYZED BY INSERTION MUTAGENESIS

渗透压波动是所有生物体的主要压力。我们一直在研究细胞对渗透胁迫的反应，以网骨藻变形虫作为模型系统。我们筛选的突变体菌落，可能有缩短表型，在渗透胁迫诱导后的随机诱变重组酶介导的质粒插入。我们建立了一种同时监测多个突变体渗透压响应的方法。通过这种方法，我们发现大约一半的筛选菌落在高渗和低渗条件下的生长和发育具有各种各样的简化表型。我们计划分析这些突变体中被破坏的基因。

Osmotic fluctuation is the major stress in all living organisms. We have been studying cellular responses to osmotic stress using Dictyostelium amoeba as a model system. We screened mutant colonies which potentially have abbreviated phenotypes in response to osmotic stress after the induction of the random mutagenesis by recombinant enzyme-mediated plasmid insertion. We established a method to monitor osmotic response in many mutants at once. By this method we found about half of the screened colonies had variety of abbreviated phenotypes in growth and development under hypertonic and hypotonic conditions. We plan to analyze destructed genes in these mutants.