Studies on control of blue-green algal growth by allelopathic effects produced by macrophyte.

在不同浓度的大型植物混合培养体系中，研究了大型植物对绿微囊藻（Microcystis aeruginosa）和其他两种蓝绿藻（Microcystis aeruginosa）生长的抑制作用，即共存试验。以棘肉豆杉（Myriophyllum spicatum）、卡罗莱纳卡龙巴（Cabomba caroliniana）等7种为大型植物。共存试验结果表明，在9种大型植物中，只有卡罗莱纳和spicatum两种植物对所有种类的蓝绿藻均有抑制作用。由于其具有较强的抑制作用，我们利用棘球蚴的培养液进行了藻类实验，证实了棘球蚴的抑制作用是由其分泌的化感物质引起的。准连续添加棘球霉培养液对铜绿假单胞菌的生长有明显的抑制作用，而初始添加对其生长没有抑制作用。这些结果提供了等位基因的证据…更多的有害化合物，必须不断地分泌m.s spicatum。接下来，我们利用高效液相色谱（HPLC）分析方法在棘棘草培养液中寻找化感物质。高效液相色谱法测定了四种化感物质（焦性没食子酸、没食子酸、(+)-儿茶素和鞣花酸）对铜绿假单胞菌具有抑制作用，且易自氧化。将各化合物与四种化合物混合后对铜绿假单胞菌的抑制作用进行了比较，结果表明，四种化合物混合后对铜绿假单胞菌具有协同抑制作用。为了验证棘球霉培养液中所鉴定的化合物是否与棘球霉化感作用有关。计算了四种化合物的释放量，以补偿培养过程中因自动氧化而造成的化合物损失，并根据计算的释放量，利用四种化合物的真实样品制备了四种化合物的人工培养液。通过对人工培养液和棘球蚴培养液的抑菌效果进行比较，可以看出这四种化合物对棘球蚴的化感作用有一定的作用。少

Inhibitory effects of macrophytes on the growth of blue-green algae (Microcystis aeruginosa and other two species) were assayed in a mixed culture system with various concentrations of macrophytes, i.e., coexistence assay. Myriophyllum spicatum, Cabomba caroliniana and other 7 species were used as macrophytes. The coexistence assay showed that, among the 9 macrophytes, C.caroliniana and M.spicatum were the only species to inhibit growth of all the species of blue green algae. The inhibitory effects of M.spicatum were stronger than those of C.caroliniana.Due to its strong inhibition, we used the culture solution of M.spicatum for an algal assay to confirm that the inhibitory effect of M.spicatum is caused by allelopathic compounds secreted from M.spicatum. The growth of M.aeruginosa was significantly inhibited by the quasi-continuous addition of the culture solution of M.spicatum, whereas the initial addition did not inhibit their growth. Such results provide the evidence of the allelop … More athic compounds, which must be continuously secreted by M.spicatum.Next, we searched for allelopathic compounds in the culture solution of M.spicatum using an analytical High Performance Liquid Chromatography (HPLC). The analytical HPLC revealed four allelopathic compounds (pyrogallic acid, gallic acid, (+)-catechin and ellagic acid), which were confirmed to be inhibitory to M.aeruginosa and susceptible to autoxidation, in the culture solution of M.spicatum. A comparison between the inhibitory effects of each compounds and those of a mixture of the four compounds showed that the mixture induced synergistic inhibitory effects on M.aeruginosa.In order to verify whether or not the identified compounds determined in the culture solution of M.spicat urn contribute to the allelopathic effects of M.spicatum, the amounts of the four compounds released by M.spicatum were calculated to compensate loss of the compounds due to auto-oxidation during the cultivation, Based on the calculated amounts, the artificial culture solution of M.spicatum was prepared using the authentic samples of the four compounds. Comparing the inhibitory effects of the artificial culture solution with those of the culture solution of M.spicatum (mentioned above), it was evident that the four compounds contributed to the allelopathic effects of M.spicatum. Less