Histological study of pathological root resorption in human deciduous teeth with apical periodontitis.

本研究的目的是探讨根尖牙周炎患者的牙根本质与正常牙根本质的不同吸收过程。用光镜和透射电镜对严重根尖牙炎患者乳牙的根尖区进行了观察。由于病理性牙根吸收，根牙本质和原牙骨质出现了严重的不规则塌陷。骨质有偏！Y沉积在塌陷的牙本质和原始牙骨质表面。沉积的骨质结构与原始骨质结构有很大的不同。人们认为牙骨质沉积等部分修复机制受根部周围环境的影响。取患有根尖牙周炎的人乳牙（p组）和健康的人乳牙（n组，对照组）分别制备牙根牙本质切片。将α MEM小鼠股骨骨髓细胞悬液接种于牙本质片上孵育。孵育后，将标本取出并固定，然后观察抗酒石酸酸性磷酸酶（TRAP）的活性。光镜下观察牙本质上trap阳性细胞的数量。所有牙本质切片均可见trap阳性细胞。经Mann-Whitney检验，p组与n组之间trap阳性细胞数差异无统计学意义。结果表明，根尖牙周炎患者的牙根质可通过培养的破骨细胞吸收，认为牙根周围的环境因素对牙根病理吸收的影响大于牙根质本身的状况。p组和n组大鼠根牙本质切片皮下包埋。两组牙本质切片均被纤维结缔组织、炎性细胞包围，部分被多核巨细胞吸收。然而，吸收是轻微的。两组牙本质切片情况无明显差异。认为牙根本质的状况对病理性牙根吸收影响不大。因此，根周环境因素对病理性根吸收有重要影响。少

The purpose of this study was to examine a different root resorption process on the root dentin with apical periodontitis or on sound root dentin.1. The region of the root apex with severe apical periodontitis in human deciduous teeth was observed by light and transmission electron microscopy. Root dentin and original cementum had a severe irregular caved surface due to pathological root resorption. Cementum had partial!y deposited on the caved dentin and original cementum surfaces. The structure of deposited cementum was very different from that of the original cementum. It was thought that partial repair mechanisms such as cementum deposition are affected by the surrounding environment of the root.2. Root dentin slices were prepared from human deciduous teeth with apical periodontitis (group p) or sound human deciduous teeth (group n, control). Cell suspension of bone marrow from the femur of mice in α MEM was seeded on the dentin slices and incubated. After incubation, specimens wer … More e removed and fixed, and then observed for tartrate-resistant acid phosphatase (TRAP) activity. The number of TRAP-positive cells on the dentin was calculated by light microscopic observation. TRAP-positive cells were observed in all dentin slices. There were no significant differences between group p and group n on the number of TRAP-positive cells by Mann-Whitney test. The results suggested that root dentin with apical periodontitis was resorbed by cultured osteoclasts It was thought that enviromental factors in root circumferences had a greater effect than does the condition of root dentin on pathological root resorption.3. The root dentin slices of group p and group n were embedded in rats subcutaneous tissue. The dentin slices of both groups were surrounded by fibrous connective tissue are inflammatory cells and partially resorbed by multinuclear giant cells. However, the resorption was slight. There were no differences between the both groups on the condition of the dentin slices. It was thought that the condition of root dentin had few effect on pathological root resorption.Therefore, it was suggested that enviromental factors in root circumferences had a great effect on patholagical root resorption. Less