D-アミノ酸の組織内分布

制备抗D-天冬氨酸(D-Asp)的多克隆抗体，通过亲和层析纯化D-天冬氨酸(D-Asp)。用该抗体对大鼠松果体进行免疫组织化学染色，发现D-Asp存在于松果体细胞的胞浆中，松果体细胞是松果体的主要细胞类型。孕12天，D-天冬氨酸在后脑、中脑和尾侧前脑有较弱的免疫反应，15~20天免疫反应增强，分布于全脑。在发育早期的未成熟前脑区域，D-天冬氨酸主要分布于神经细胞的胞浆中，而在发育较成熟的后脑中，D-天冬氨酸主要分布在轴索区。在发育后期(E18)分化程度较高的前脑，IR主要局限于以轴突和突起为主的区域。在睾丸中，D-天冬氨酸免疫反应在生殖细胞的细胞质中很明显，尤其是在生殖细胞中最成熟的细长精子细胞的富集区。精母细胞和圆形精子细胞的胞浆内也有微弱的IR。

Specific polyclonal antibody was raised against D-aspartate (D-Asp) which had been conjugated to glutaraldehyde and was purified by affinity chromatography. Immunohistochemical staining of rat pineal gland with the antibody demonstrated the presence of D-Asp in the cytoplasm of pinealocytes, the predominant cell type in this gland. At gestational day (E) 12, weak immunoreactivity (IR) of D-Asp was apparent at the hindbrain, midbrain and caudal forebrain, whereas it became more intense and extended over the whole brain at 15 20. In the region of the immature forebrain at an early stage of development (E12), IR was mainly a characteristic of the cytoplasm of the neuronal cells, while in the more mature hindbrain it was localized in the axonal zone. In the more differentiated forebrain at a later stage of development (E18), the IR became restricted to zones which mainly consisted of axons and processes. In the testis, D-Asp IR was evident in the cytoplasm of germ cells, especially around the region rich in elongate spermatids, the most mature of the germ cells. Weak IR was also noted in cytoplasm of spermatocytes and round spermatids.