Structure, function, and dynamics of the TOC core complex (P17)

蛋白质跨膜转运是细胞功能的核心，涉及特定的膜整合蛋白复合物。外塑料膜（TOC）的转座子由至少四个亚基组成——三个受体蛋白Toc34、Toc64和Toc159，以及桶状通道Toc75。虽然TOC的整体功能已被描述，但在分子和原子水平上的机制尚不清楚。除了将Toc34和Toc159的GTPase周期作为易位的核心部分进行表征外，我们还将对整个TOC复合体进行结构分析。这些信息将与探索该复合物在不同发育阶段和组织中的动力学相结合，为一般易位过程的分子理解提供基础。

Protein translocation across membranes is central for cellular function and involves specific membrane integral proteinaceous complexes. The translocon of the outer plastidic membrane (TOC) is composed of at least four subunits - the three receptor proteins Toc34, Toc64 and Toc159, and the barrel shaped channel Toc75. While the global function of the TOC is described, not much is known about the mechanisms at molecular and atomistic levels. We will move toward a structural analysis of the entire TOC complex in addition to the characterization of the GTPase cycle of Toc34 and Toc159 as central part for the translocation. This information will be unified with the exploration of the dynamics of this complex in different developmental stages and tissues to provide a basis for a molecular understanding of the translocation process in general.