Molecular and biophysical approaches to study insulin secretory granules and lipid microdomains in vitro and in living insulinoma cells

ICA512是胰岛素分泌颗粒（SGs）的受体，其胞浆尾部在SG胞吐时断裂。这一事件触发了一个信号级联反应，允许胰腺ß-细胞根据代谢需求调整胰岛素释放、SG生物发生和增殖。我们计划将分子和生化方法与全内反射显微镜和荧光相关光谱相结合，定量地观察单个ICA512分子的裂解与膜微域的关系。这些信息可能有助于我们理解糖尿病和膜微结构域与激素分泌和细胞信号传导有关的动态性质。

ICA512 is a receptor of the insulin secretory granules (SGs) whose cytosolic tail is cleaved upon SG exocytosis. This event triggers a signalling cascade that allows pancreatic ß-cells to adjust their insulin release, SG biogenesis and proliferation to metabolic demand. We plan to combine molecular and biochemical approaches with total internal reflection microscopy and fluorescence correlation spectroscopy to quantitatively visualize the cleavage of single ICA512 molecules in relationship to membrane microdomains. This information may benefit our understanding of diabetes and of the dynamic nature of membrane microdomains in relation to hormone secretion and cell signaling.