The suppression of allograft rejection in retinal pigment epithelial transplantation with ACAID.

多种抗原接种到眼睛前房（AC）可以引起T细胞介导的延迟型超敏反应（DTH）的全身抑制。这种现象被称为前房相关免疫偏差（ACAID）。在本研究项目中，我们首先研究了ACAID是否可以通过诱导抑制角膜异体移植排斥反应。从B10收集脾细胞。将D2小鼠注射到BALB/c小鼠的AC中。7 d后，用B10皮下免疫BALB/c小鼠。D2脾细胞。7天后，通过耳部肿胀反应评估DTH。BALB/c小鼠眼内移植B10。使用评分系统评估D2角膜小体和同种异体上皮的排斥反应。结果表明，AC接种脾细胞可诱导DHT抗原特异性反应，并抑制上皮排斥反应。与AC接种脾细胞相比，AC接种来自Lewis大鼠和B10的视网膜色素上皮（RPE）。D2小鼠未诱导ACAID。我们的结果表明RPE不可能是视网膜中的抗原提呈细胞。

A variety of antigens inoculated into the anterior chamber (AC) of the eye can elicit systemic suppression of T cell-mediated delayed-type hypersensitivity (DTH). This phenomenon is called anterior chamber-associated immune deviation (ACAID). In this research project, we first examined whether corneal allograft rejection can be suppressed by the induction of ACAID. Splenocytes collected from B10.D2 mouse were injected into the AC of BALB/c mouse. Seven days later, BALB/c mice were immunized subcutaneously by B10.D2 splenocytes. Seven days later, DTH was evaluated by ear swelling response. The eyes of BALB/c mice were grafted with B10.D2 corneal lenticles and alloepithelial rejection was evaluated using the scoring system. The results showed that AC inoculation of splenocytes induced antigen-specific of DHT response as well as supression of epithelial rejection.In contrast to AC inoculusion of splenocytes, AC inoculusion of retinal pigment epithelium (RPE) collected from Lewis rat nor B10.D2 mouse did not induce ACAID. Our results suggested that RPE could not be antigen presenting cell in retina.