p53 gene mutation in Japanese patients with esophageal

对于胸段食道癌，我们进行了扩大的三野淋巴结清扫术，获得了近50%的总5年生存率。然而，患者在常规组织病理学检查未发现淋巴结转移的情况下，有时仍会发生淋巴结复发。在一例食管鳞癌患者中，我们对原发癌的所有P53基因编码区(外显子2-10)进行了测序，证实了1例P53外显子10的无义突变。然后从该患者的150个手术切除的淋巴结中提取基因组DNA，并用聚合酶链式反应分析(PCR-RFLP)检测淋巴结中相同的P53突变，发现6个淋巴结中有相同的P53突变，其中1个位于右侧喉返神经旁，常规组织病理学检查未发现阳性结节，转移癌的P53突变诊断可能有助于微小残留病变的检测。

For thoracic esophageal cancer, we perform extended three field lymph node dissection, and have achieved nearly 50% of overall 5-year survival.However, patients sometimes develop lymph node recurrences in spite of having no lymph node metastases found by conventional histopathologic examination.In a patient with esophageal squamous cell carcinoma, we sequenced all the p53 cDNA translated regions (exon 2 - 10) of primary carcinoma, and confirmed one p53 nonsense mutation in exon 10.Then we extracted genomic DNA from 150 surgically dissected lymph nodes from that patient, and performed polymerase chain reaction analysis (PCR-RFLP) to detect the same p53 mutation in the lymph nodes.PCR-RFLP analysis showed the same p53 mutation in six lymph nodes.One node was located along the right recurrent laryngeal nerve, where no positive nodes was identified by conventional histopathologic examination.The p53 mutational diagnosis of metastatic cancer may be useful in detecting minimal residual disease.