A study of the mechanism of immune induced central nervous system anomaly

抗孕大鼠肾血清（ARKS）致中枢神经系统异常。为了阐明免疫异常的发生机制，我们对几类免疫异常进行了研究。与癌基因相关的研究结果，我们不能证明s-myc、c-、nyc、fas和RB的表达，只有野生型P53的表达，并认为该抑制基因可能被ARKS诱导修复受损的细胞核。注射生理盐水的对照组Mib-1和Ki-67均不表达，仅PCNA表达高于注射ARKS的对照组。结果表明，ARKS抑制神经管上皮细胞的生长。未发现染色体异常。如果你可以考虑怀孕是器官移植之一，有一些细胞介导的免疫作用。本研究探讨了该模型与细胞免疫的关系。T细胞、B细胞和 ...更多信息 巨噬细胞在ARKS注射组大鼠子宫、胎盘、羊膜中均有表达。应用荧光免疫组织化学方法，观察了两种凝集素--刀豆球蛋白A（ConA）和麦胚凝集素（WGA）与神经上皮糖缀合物的结合。发现妊娠第9天（GD 9）的胚胎，在神经上皮细胞间区的腔侧和神经管的基底膜上可见荧光。在GD 21胚胎中，注射ARKS的大鼠胚胎在神经形成期神经上皮细胞表面的细胞外基质和室管膜衬里细胞的荧光强度明显高于注射NRS的正常胚胎，这些结果支持了单纯的不闭合和过度生长是神经管缺陷的机制。中枢神经系统异常表达与细胞凋亡的关系目前尚不清楚。因为母胎组织中fas和bcl-2的免疫组化检测均为阴性。少

The central nervous system anomalies induced by anti-pregnant rat kidney serum (ARKS). In order to clarify the mechanism of immune induced anomalies in rat, we studied several numbers of categories. The results of study related to oncogene, we could not demonstrate an expression of s-myc, c-, nyc, fas and RB.Only wild type of P53 was expressed, and it was considered that this supressor gene may be induced to repair the damaged nuclei by ARKS.On the tumor growth factor, Mib-l and Ki-67 did not express and only PCNA revealed higher in control which were injected saline instead of antiserum than ARKS injected rats. This results suggested that the ARKS inhibit the growth of epithelial cells of the neural tube. The abnomality of chromosome was not detected at this time. If you may consider that the pregnancy is one of organ transplantation, there is some action of cell mediated immunity. We studied the relation of cell mediated immunity in this rat model. The antigens of T-cell, B-cell, and … More macrophages were demonstrated in uterus, placenta, amunion of ARKS injected rats group. The cell mediated immunity did not act directry but act indirectry to the fetus which treated by ARKS.Fluorescence immunohistochemical studies were performed to localize the binding of two lectins, concanavalin A (Con A) and wheat germ agglutinin (WGA), to glycoconjugates on neuroepithelium. We found fluorescence was observed on the luminal side of the neuroepithelium in the intercell- ular region and on the basement membrane of the neural tube in embryos on day 9 of gestation (GD 9). In GD 21 embryos there was much more intense fluorescence in the extracellular matrix and the ependymal lining cells of the cell surface of the neuroepithelium during neurulation was different in rat embryos injected with ARKS than in normal embryos injected with NRS.These results support the idea that simple nonclosure and overgrowth constitute the mechanism of neural tube defect. The relation of apoptosis in expression of CNS anomaly is not clear at present. Because, fas and bcl-2 was totally negative in immunohistochemical examinations on the both mother and fetal tissues. Less