The study Epstein-Barr virus on the disease progression in patients with idiopathic interstitial pneumonia.

EB病毒（EBV）与特发性肺纤维化（LPF）的相关性在最近的文献中存在争议。本研究的目的是阐明病毒的存在以及EB病毒对IPF临床特征的影响。制备了20例IPF患者、5例系统性硬化症伴肺纤维化（SSc）患者和15例正常对照（NC）肺标本。应用两步法PCR和免疫组化方法检测EB病毒DNA和EB病毒潜伏膜蛋白1（LMP 1）。分别两步PCR法在IPF患者肺组织中的检出率明显高于NC患者（IPF组96%vsNC_4组71%，P<0.05）。免疫组化结果显示，29例IPF患者中9例肺立方上皮细胞呈抗LMP 1和抗LMP 1抗体阳性。相反，SSc患者和NC均未显示阳性染色。重要的是，LMP 1阳性IPF患者在“疾病进展”中具有更高的频率，“疾病进展”定义为在随后的时期内PaO 2降低打鼾超过15 μ g，而LMP 1阴性患者：7名LMP 1阳性患者中的5名对13名LMP 1阴性患者中的1名; p<0.01。我们的结论是，EBV通过表达LMP 1参与了肺纤维化的进展，至少在一些IPF患者中是这样。

The relevance of Epstein-Barr virus (EBV) for idiopathic pulmonary fibrosis (LPF) is controversial in recent literatures. Our aims in this study are to elucidate the existence of the virus as well as to clarify the influences of EBV to the clinical features of IPF.Twenty-time lung specimens from IPF patients and 5 from systemic sclerosis patients with puImonary fibrosis (SSc) and 15 from normal controls (NC) were prepared. Detection of EBV DNA and EBV latent membrane protein-1 (LMP1) were analyzed using two-step PCR methods and immunohistry. respectively. The detection ratio was significantly higher in the lungs with IPF patients than in those with NC by two-step PCR methods (96% in IPF vs 71% in NC_4 P<0.05). ImmunohistochemieaI analysis reveded taht cuboidai epithelial cells were positively stained with and-LMP1 andbody in 9 of 29 lungs front IPF patients. In contrast, none of the SSc patients and NC showed positive staining, It was significant that LMP I -positive IPF patients had more freqnency in "disease progression", defined as if PaO2 decreased snore than 15 torr during foIIow up periods, titan LMP1 -negative paIienis : 5 of 7 LMPI-positive vs 1 of 13 LN'IP1-negative patients ; p<0.01. We concluded that EBV was involved in the progression of the lung fibosis by expressing LMP1, at least, in some of the parients with IPF.