Making and analysis of a transjenic model of Mastocytosis and urticalia pigmentosa by the forced expression of steel factor in the skin.

通过在皮肤中强制表达钢因子，建立了肥大细胞增多症和色素性荨麻疹转基因小鼠模型，并获得了以下结果.肥大细胞增多症可重复地通过可溶性钢因子在皮肤基底细胞中的表达来表达。膜结合型钢因子只发生了黑变病，而没有任何肥大细胞增生的迹象.肥大细胞增多症的小鼠均出现皮肤黑素细胞过度生长，表现为肥大细胞增多症最常见的色素性荨麻疹症状.在体内施用钢因子-c-Kit信号传导阻断抗体完全消除了肥大细胞和黑素细胞的维持，表明这些细胞的增加是由钢转基因的表达引起的.我们的肥大细胞增多症模型显示更快和更广泛的炎症反应。这进一步意味着模型小鼠将用于研究肥大细胞在炎症中的作用。

Transgenic mouse models for mastocytosis and urticalia pigmentosa were made by the forced expression of steel factor in the skin and the following results were obtained.1. Mastocytosis was reproducibly expressed by the expression of soluble steel factor in the basal cells of skin. Membrane-bound type steel factor developed only melanosis, but not any signs of mastocytosis.2. The mice that had shown mastocytosis were all developed melanocyte overgrowth in the skin, representing the symptom of urticalia pigmentosa, most common types of mastocytosis.3. In vivo administration of blocking antibody of steel factor-c-Kit signaling completely abolished the maintenance of mast cells and melanocytes, indicating that increase of these cells were caused by the expression of the steel transgene.4. Our mastocytosis models showed quicker and more extensive inflammatory response. This further implies that the model mice will be useful for the study of the roles of mast cells in inflammation.