Physiological roles of insulin-like growth factor I in the mouse uterine endometrium.

雌激素和孕激素调节子宫内膜细胞的生长。雌激素诱导的小鼠子宫内膜细胞增殖和分化可能是由生长因子介导的。本研究旨在探讨胰岛素样生长因子(IGF-I)在原代无血清培养条件下对小鼠上皮细胞和基质细胞增殖的影响。在上皮细胞和间质细胞中均检测到IGF-I和IGF-I受体(r型)的mRNA。雌激素刺激IGF-I的合成，雌孕激素联合在基因转录水平刺激IGF-I受体的合成。IGF-I以剂量依赖的方式刺激上皮细胞和基质细胞的增殖。这些研究表明，子宫内膜IGF-I以旁分泌或自分泌的方式调节子宫内膜细胞的生长，很可能是作为雌激素作用的介体。将基质细胞悬浮在胶原凝胶中，将上皮细胞种植在胶原凝胶的基质细胞层上。上皮细胞内可见连接复合体结构(紧密连接和带状粘连)和微绒毛。上皮细胞与基质细胞共培养可增加培养的上皮细胞数量。这表明基质细胞影响上皮细胞的生长。上皮细胞和间质细胞之间这种相互作用的分子机制尚需阐明。

Estrogens and progestin regulate the growth of uterine endometrial cells. Estrogen-induced proliferation and differentiation of mouse endometrial cells may be mediated by growth factors. This study was undertaken to determine whether insulin-like growth factor (IGF-I)' stimulates proliferation of mouse epithelial and stromal cells in primary serum-free culture. In both epithelial and stromal cells, IGF-I mRNA and IGF-I receptor (type r) mRNA were detected. Estrogen stimulated IGF-I synthesis, and combination of estrogen and progesterone stimulated IGF-I receptor synthesis at the level of gene transcription. IGF-I stimulated the proliferation of both the epithelial and stromal cells in a dose-dependent manner. These study altogether indicate that endometrial IGF-I regulates the growth of endometrial cells in a paracrine or autocnine manner, highly probably as mediator of estrogen actions.We successfully reconstituted the uterine endometrium in vitro. The stromal cells were suspended in collagen gel, and the epithelial cells were plated on the stromal cell layer in the collagen gel. Junctional complex structure (tight-junction and zonula adherence) and microvilli were observed in the epithelial cells. Co-culture of epithelial cells and stromal cells increased the number of epithelial cells cultured. This suggests that the stromal cells affect the growth of epithelial cells. Molecular mechanism of this interaction between the epithelial cells and stromal cells needs to be clarified.