DEVELOPMENT OF NATURAL MEDICINES FROM THE NEW WORLD PLANTS

1)参考：1998年7月，南俄勒冈大学的朗教授应邀到日本鉴定1997年实地考察获得的植物标本。讨论了植物粗提物抑制HIV生长和抗肿瘤活性(KB细胞)的筛选试验结果。2)野外调查：1997年7月和8月分别在美国西海岸、危地马拉和洪都拉斯进行了野外调查，1998年7月和8月在墨西哥、危地马拉和洪都拉斯进行了实地调查。共采集植物样品176个，市售中药材30个，标本馆标本800个。3)提取液的制备和生物学测试：在Setsunan大学、岐阜市药科大学和岐阜市卫生与环境科学研究所，将植物样品分为根、茎、叶、果实等，然后用丙酮、甲醇、70%甲醇和水等各种溶剂进行提取。共获得800个提取物，用于3T3-L1细胞的HIV生长抑制、抗肿瘤活性、抗菌活性的生物学试验和对脂肪细胞分化的调控化合物的筛选。金鱼藤科、千金藤科、藜科、茄科、野牡丹科、灯盏花科、唇形科、锦葵科、金缕梅科等植物的提取物对HIV的生长有明显的抑制作用。部分研究结果发表在科学论文上。在抗肿瘤活性(KB细胞)的筛选试验中，从番荔枝科、柏科、大戟科、唇形科、胡椒科、香菜科、远志科、茜草科、茉莉科、菊科、豆科、锦葵科等植物的提取物中发现了显著的抗肿瘤活性。

1)Inviation : In July 1998, Prof. Lang of Southern Oregon University was invited to Japan to identify the plant specimens obtained by the field study in 1997. Results of the screening tests of HIV growth inhibition and antitumor activity (KB cells) for the crude plant extracts were discussed. Based on the results, plants for collection in the field research in 1998 were planned.2)Field research : Field researches were canied out in the west coast of USA, Guatemala and Honduras in July and August 1997 and in Mexico, Guatemala and Honduras in July and August 1998, respectively. Totall 176 plant samples and 30 herbal market products for extraction, and 800 herbarium specimens were collected.3)Preparation of extracts and biological test : In Setsunan University, Gifu Pharmaceutical University and Gifu Prefectural Institute of Health and Environmental Sciences, plant samples were divided into roots, stems, leaves and fruits, etc., then extracted with various solvents such as acetone, methanol, 70% methanol and water. Total 800 extracts obtained have been used for biological tests of HIV growth inhibition, anti-tumor activity, antibacterial activity and screening compaounds regulating the differentiation into adipocyte in 3T3-L1 cells. Remarkable HIV growth inhibition was observed in extracts from Onaglaceae, Lythraceae, Chenopodiaceae, Solanacace, Melastomataceae, Ebenaceae, Labiatae, Malvaceae and Monimiaceae. A part of the results was published in the scientific papers. In screening tests of anti-tumor activity (KB cells), significant activities were found in extracts from Annonaceae, Cupressaceae, Euphorbiaceae, Labiatae, Piperaceae, Polemoniaceae, Polygaraceae, Rubiaceae, Meliaceae, Compositae Leguminosae, Malvaceae, etc. Of the bioactive extfacts suggested by these tests, some are now under components analysis.