Molecular mechanisms of ion transport and clinical application

1. Pancreatic HCO-D23-D2-D1-D1 and fluid secretion were studied by monitoring luminal pH and luminal volume simultaneously in interlobular duct segments （diameter ~ 100 mm） is isolated from guinea-pig pancreas。We found that HCOイD23イD2イD1-イD1 uptake across the basolateral·brane is achieved largely by NaイD1+イD1-HCOイD23イD2イD1 cotransport， and that HCOイD23イD1-イD1 efflux and fluid secretion across the luminal·brane involve HCOイD23イD2イD1-イD1 conductance。The Location of Purinoceptors in pancreatic duct cells及其在regulating HCO D23 y D2 y D1-y D1 and fluid secretion被研究在microperfused duct segments中。We found that both apical and basolateral nucleotides inhibit it。The efficients of arginine vasopressin （AVP） on ductal secretion were studied in isolated vascularly perfused ductal fluid secretion via V-D21-D2 receptors. 2。我们已经采用分子方法来研究水运输和克隆一种新的水生植物，从大鼠的肝脏中提取水。 ... More 氨基酸序列识别为人类AQP9.3。我们也在研究水和电细胞在体内的运输。我们发现了一种被管理的guanylin和热稳定的电子毒素，导致了流体秘密的光芒。Intravenous guanylin induced mucus secretion from goblet cells in rat duodenal crypts.4.我们在有意识的狗中对泛血流动和泛血保密进行了连续和相似的测量。我们发现了pituitary adenylate cyclase激活肽（PACAP）和vasoactive intestinal peptide （VIP）诱导的vasodilatation可以与观察后的vasodilatation进行比较。We have started to investigate the relationships between mutations in cystic fibrosis transManagement brane conductance regulator （CFTR） and？ic pancreatitis in Japan. 6。We examined the effManagement of phospholipase AイイD22イD2 （PLAイD22イD2） inhibitors on CaイD12+イD1 oscillations and amylase secretion in rat pancreatic acinar cells。We found that type IV （cytosolic）PLAイイD22イD2 regulates CaイD12+イD1 signaling and enzyme secretion。Less（低）

1. Pancreatic HCOィイD23ィエD2ィイD1-ィエD1 and fluid secretion were studied by monitoring luminal pH and luminal volume simultaneously in interlobular duct segments (diameter〜100mm) is isolated from guinea-pig pancreas. We found that HCOィイD23ィエD2ィイD1-ィエD1 uptake across the basolateral membrane is achieved largely by NaィイD1+ィエD1-HCOィイD23ィエD2ィイD1-ィエD1 cotransport, and that HCOィイD23ィエD2ィイD1-ィエD1 efflux and fluid secretion across the luminal membrane involve HCOィイD23ィエD2ィイD1-ィエD1 conductance. The location of purinoceptors in pancreatic duct cells and their role in regulating HCOィイD23ィエD2ィイD1-ィエD1 and fluid secretion was studied in microperfused duct segments. We found that both apical and basolateral nucleotides inhibit it. The efficients of arginine vasopressin (AVP) on ductal secretion were studied in isolated vascularly perfused ductal fluid secretion via VィイD21ィエD2 receptors.2. We have adopted molecular methodologies to study water transport and cloned a new aquaporin from rat liver with high … More amino-acid sequence identify to human AQP9.3. We also conduct study of water and electrolytes transport in vivo. We found that guanylin and heat-stable enterotoxin administered into the lumen of rat jejunum caused fluid secretion. Intravenous guanylin induced mucus secretion from goblet cells in rat duodenal crypts.4. We have succeeded in a continuous and simultaneous measurement of pancreatic blood flow and pancreatic secretion in conscious dog. We found that pituitary adenylate cyclase activating peptide (PACAP) and vasoactive intestinal peptide (VIP) induced vasodilatation comparable to that observed after a meal.5. We have started to investigate the relationships between mutations in cystic fibrosis transmembrane conductance regulator (CFTR) and chronic pancreatitis in Japan.6. We examined the effects of phospholipase AィイD22ィエD2 (PLAィイD22ィエD2) inhibitors on CaィイD12+ィエD1 oscillations and amylase secretion in rat pancreatic acinar cells. We found that type IV (cytosolic)PLAィイD22ィエD2 regulates CaィイD12+ィエD1 signaling and enzyme secretion. Less