The regulation of gut-brain communication during Caenorhabditis elegans dauer development

项目摘要 不同组织（或器官）之间的通讯，如肠-脑轴，在调节 动物生理学和发育决策。线虫秀丽隐杆线虫进入一个非进食， 发育停滞的dauer阶段，以科普恶劣的环境，如食物短缺和过度- 人口Dauer决策受多种途径（胰岛素、生长因子和激素）调节， 涉及神经系统、XXX神经内分泌细胞和肠。先前的研究已经建立了 C.线虫作为模式生物研究应激反应的发展，但组织特异性调节 Dauer的发展仍不清楚。特别是，很少有人知道神经系统如何协调， 肠道整合信号通路，并作出整个有机体的dauer决定。拟议 目的是提供一个系统的研究，不同的组织如何通过多个 信号通路，并利用分子遗传学工具协调调节dauer决策 最近在赞助商实验室研发的为了实现这一目标，Aim 1将重点调查 每个组织在dauer发展过程中的作用，并建立调控网络的模型;目标2将侧重于 每个组织和每个通路在Dauer决策启动和执行中的时间参与，还 开发出精确的道尔调节电路。总之，拟议的目标将扩大目前的 了解滞育发生时间的分子机制 to environmental环境stress应力.利用C. elegans dauer决定作为一个模型，建议的项目将有助于 更好地了解组织间的协调，特别是在脑肠道通信，在 发展决策过程。

Project Summary The communication among different tissues (or organs), such as the gut-brain axis, is crucial in regulating animal physiology and developmental decisions. The nematode Caenorhabditis elegans enters a non-feeding, developmentally arrested dauer stage to cope with the harsh environments, such as food shortage and over- population. The dauer decision is regulated by the multiple pathways (insulin, growth factor, and hormone), involving the nervous system, XXX neuroendocrine cells, and intestine. Previous research has established C. elegans as a model organism to study stress-responsive development, but tissue-specific regulation of dauer development remains unclear. In particular, little is known about how nervous system coordinates with the intestine to integrate the signaling pathways and make a whole-organism dauer decision. The proposed aims focus on providing a systematic study of how different tissues communicate through multiple signaling pathways and coordinately regulate the dauer decision using molecular genetics tools recently developed in the sponsor lab. To achieve this goal, Aim 1 will focus on investigating the general roles of each tissue during dauer development and build models for the regulatory network; Aim 2 will focus on temporal participation of each tissue and each pathway in dauer decision initiation and execution, further developing precise circuits of dauer regulation. Altogether, the proposed aims will expand the current knowledge of molecular mechanisms underlying developmental timing of commitment to diapause in response to environmental stress. Using C. elegans dauer decision as a model, the proposed project will contribute to better understanding of the inter-tissue coordination, particularly in brain-gut communication, during the developmental decision making process.