Administrative Supplement To Incorporating the Microbiome into DR2 Activities to Inform Health Outcomes

项目摘要 飓风哈维之后发生在休斯顿的创纪录洪水造成了不受控制的 从十几个超级基金站点和几个化学品/石油释放到环境中 设施在我们的时间敏感的R21父，我们与受影响的社区， 管理健康问卷，部署腕带以检测化学品暴露，以及 在洪水后1个月和12个月收集生物样本进行微生物组分析。在这些最初的 我们发现了一种新的微生物组：与过敏性健康相关的环境相互作用 结果。根据本行政补充文件，我们将在这一工作的基础上，纵向开展工作- 哈维飓风过后三年，对鼻腔、口腔和肠道生物标本进行了灵敏的采样。这些 生物标本和微生物组数据将用于持续的灾害研究响应（DR 2） 活动，并作为未来微生物组的独特资源存储：环境相互作用研究。 它们还将与从腕带监测中获得的化学暴露数据相结合 设备和健康结果数据，来自研究参与者的问卷调查， 对应的时间点。我们的总体假设是微生物组：环境 相互作用在洪水灾害后的长期健康影响中发挥作用。如果正确的话， 这将进一步支持微生物组可以作为这种环境的目标。 风险暴露或可采取行动的灾后活动指标。在具体目标1中，我们将收集 洪水后3年时间点的参与者的环境和生物样本， 扩大我们的生物样本收集，包括反映长期健康的样本 结果。在具体目标2中，我们将通过宏基因组测序和测试来分析这些样本， 无论是早期发现的微生物组暴露或微生物组健康结果关系， 时间点长期存在。这些包括鼻真菌生物群与 在家里发现的真菌和肠道微生物群落之间的关系，霉菌 暴露和过敏症状。最后，在具体目标3中，我们将整合1个月、12个月 和3年的微生物组数据，以及短期、中期和长期健康评估， 我们的合作者正在进行化学暴露研究， 微生物组：环境相互作用。我们将询问微生物组分析是否来自 短期、中期和长期收集的生物样本与化学品暴露相关 通过腕带或其他环境监测捕获，并共同告知健康结果。在 在未来，这些研究有望为旨在1）更好地 了解微生物组对灾后健康结果的作用，2）确定 暴露和/或健康影响的基于微生物组的生物标志物，以及3）改善决策- 利益相关者的决策和DR 2活动，以应对未来的洪水相关灾害。

PROJECT ABSTRACT The record flooding that occurred in Houston after Hurricane Harvey caused uncontrolled releases into the environment from over a dozen Superfund sites and several chemical/petroleum facilities. Under our Parent Time-sensitive R21, we worked with affected communities to administer health questionnaires, deployed wristbands to detect chemical exposures, and collected biosamples for microbiome analysis at 1- and 12-months after flooding. In these initial studies we identified a novel microbiome:environment interaction linked to allergic health outcomes. Under this Administrative Supplement, we will build on this work with longitudinal time- sensitive sampling of nasal, oral and gut biospecimens three years post-Hurricane Harvey. These biospecimens and microbiome data will be used for continuing disaster research response (DR2) activities, and stored as a unique resource for future microbiome:environment interaction studies. They will also be integrated with chemical exposure data obtained from wristband monitoring devices, and health outcome data from questionnaires administered to study participants at corresponding timepoints. Our Overarching Hypothesis is that microbiome:environment interactions play a role in the long-term health effects following a flood-related disaster. If correct, this would further support that the microbiome can serve as a target for such environmental exposures, or an indicator for actionable, post-disaster activities. In Specific Aim 1, we will collect environmental and biological samples from participants at the 3-year timepoint post-flooding, expanding our biospecimen collection to include samples reflective of a longer-term health outcomes. In Specific Aim 2, we will profile these samples via metagenomic sequencing and test whether microbiome-exposure or microbiome-health outcome relationships identified at earlier timepoints persist long-term. These include continued associations of the nasal mycobiome with fungi found in the home and the relationship between the gut microbial community, mold exposure, and allergic symptoms. Finally, in Specific Aim 3 we will integrate 1-month, 12-month and 3-year microbiome data with short- intermediate- and long-term health assessments and chemical exposure studies being conducted by our collaborators to identify novel microbiome:environment interactions. We will ask whether microbiome analyses from biospecimens collected over short-, medium-, and long-term correlate with chemical exposures captured by wristbands or other environmental monitoring and jointly inform health outcomes. In the future, these studies are expected to provide Preliminary Data for research aimed at 1) better understanding the role of the microbiome on post-disaster health outcomes, 2) identifying microbiome-based biomarkers of exposure(s) and/or health effects, and 3) improving decision- making by stakeholders and DR2 activities in response to future flood-related disasters.