The ShEEP request for NanoString GeoMx Digital Spatial Profiling and nCounter MAX Complete System

此SHEEP应用程序申请资金以购买完整的NanoStringGeoMx®Digital系统 将用于支持弗吉尼亚州中部退伍军人管理局研究的空间概况分析和NCounter®Max 医疗保健系统。退伍军人事务部资助的九名主要调查人员，包括周博士，希利蒙，帕特尔，王， Bajaj，Ren，Pandak，Gartenhaus和Ghosh需要这种设备来测量mna，miRNA，lncRNA 和蛋白质在细胞、新鲜或冷冻组织、血清和胆汁中的表达 以及美国国立卫生研究院的拨款。请求的NanoString的GeoMx®数字空间剖面图技术可以检测和 使用抗体或RNA以更高的空间分辨率对蛋白质和RNA进行定量 连接到可光切割的DNA寡核苷酸的探针，用Ncount®Max读数进行异位定量 福尔马林固定石蜡包埋(FFPE)或新鲜冰冻切片上的特定感兴趣区(ROI) 组织。可变ROI选择允许对直径为700-μm的区域进行分析，精确到单个单元格(10- µm)。不需要二次抗体或扩增试剂。GeoMx®数字读数 Space Profiler由全自动且易于使用的NCounter®Max分析系统执行。 NCounter®Max提供了在不到24小时内经济高效地完成项目所需的一切 几个小时。比定量聚合酶链式反应更准确、更具重复性和高度多样性，而且更简单、更快速、更多 与NGS相比，NCounter®MAX更具成本效益，它允许在 在无需提取的工作流程中使用单管，只需要15分钟的实际操作时间。这允许 核心设施，通过在样品准备和自我驱动方面花费更少的时间来加速研究， 使用开放使用的nSolver®分析软件进行简单、快速的数据分析，这是 协作工作。直观的工作流程，从样本到数据只需15分钟的实际操作时间。 独立的数字分析仪和准备站单元有助于消除样品处理和处理的瓶颈 数据收集。不需要专门的生物信息学家。以直接数字计数生成的结果和 在标准CSV文件中报告，该文件可以导入到任何常用的应用程序中，也可以使用随附的 NSolver®分析软件。为满足以下要求的实验室提供了企业软件包 增强的安全性。控制用户访问、自动执行数据流并生成审核日志。目前，没有 我们的退伍军人研究中心也有类似的设备。调查人员依靠外界的力量 合同服务，这不仅是昂贵和耗时的，而且还需要生物信息学的支持。 鉴于分子变化(RNA、DNA、miRNA和蛋白质表达)在生物学中的关键作用 在整个发育和疾病过程中，这种设备将使我们的研究人员能够促进 现有的研究，并以无法达到的效率和精确度追寻未知的研究领域 由研究设施中现有的仪器设备实现。因此，我们向绵羊申请资金 购买此系统用于我们正在进行的与代谢性肝病相关的VA资助研究 是退伍军人事务部研究任务的目标区域。收购这一最先进的系统将大大 扩大我们在基础研究、转化研究和临床研究方面的核心能力，并促进 退伍军人事务部资助的基础研究科学家和内科科学家之间的合作。此外，它还将 为支持初级调查人员生成可靠的初步数据提供出色的平台 竞相争取外部拨款。这些由退伍军人事务部资助的研究的成功完成不仅将 使美国退伍军人医疗保健受益，但也使所有美国人受益。

This ShEEP application requested funds to purchase a complete system of NanoString GeoMx® Digital Spatial Profiling and nCounter® MAX that will be used to support VA research at the Central Virginia VA Health Care System. Nine major VA-funded investigators, including Drs. Zhou, Hylemon, Patel, Wang, Bajaj, Ren, Pandak, Gartenhaus, and Ghosh require this equipment to measure mRNA, miRNA, lncRNA and protein expression in cells, fresh or frozen tissues, serum and bile for their funded VA Merit Awards and NIH grants. The requested NanoString’s GeoMx ® Digital Spatial Profiling technology can detect and quantify proteins and RNA at significantly higher plex with spatial resolution using antibodies or RNA probes conjugated to photo-cleavable DNA oligos that are quantified ex situ with nCounter® MAX readout from specific regions of interest (ROI) on Formalin-Fixed Paraffin-Embedded (FFPE) or Fresh Frozen tissue. Variable ROI selection permits the profiling of 700-μm diameter areas down to single cells (10- µm). No secondary antibodies or amplification reagents are required. Readout of the GeoMx® Digital Spatial Profiler is performed by the fully automated and easy-to-use nCounter® MAX Analysis System. The nCounter® MAX provides everything needed to cost-effectively complete projects in less than 24 hours. More accurate, reproducible and highly multiplexed than qPCR and simpler, quicker and more cost-effective than NGS, the nCounter® MAX allows for digital examination of multiple pathways in a single tube in an extraction-free workflow requiring only 15 minutes of hands-on time. This allows the Core facility to accelerate research by spending less time on sample prep and performing self-driven, simple, and rapid data analysis using the open use nSolver® Analysis Software, a great tool for collaborative work. The intuitive workflow with only fifteen minutes of hands-on time from sample to data. Separate Digital Analyzer and Prep Station units help eliminate bottlenecks in sample processing and data collection. No need for a specialized Bioinformaticist. Results generated as direct digital counts and reported in a standard CSV file that can be imported into any favorite application or use the included nSolver® Analysis Software. An enterprise software package is included for laboratories that require enhanced security. Control user access, automate data flow and generate audit logs. Currently, no similar equipment is available at our VA Research facility. The investigators are relying on outside contract services, which are not only costly and time-consuming but also require Bioinformatics’ support. Given the critical role of the molecular changes (RNA, DNA, miRNA and protein expression) in biological processes throughout development and disease, this equipment would allow our investigators to facilitate existing studies and pursue unexplored areas of research with efficiency and accuracy that could not be achieved by the current instrumentation in the research facility. Therefore, we request funds from ShEEP to purchase this system for use in our ongoing VA-funded studies related to metabolic liver diseases that are target areas of the VA research mission. Acquisition of this state-of-the-art system will significantly expand our Core capacity for both basic research, translational and clinical studies and promote collaborations between VA-funded basic research scientists and physician-scientists. Furthermore, it will provide an excellent platform for supporting junior investigators to generate solid preliminary data for competing for external grants. The successful completion of these VA-funded research will not only benefit US Veterans health care but also benefit all Americans.