A Comprehensive Human cDNA Library For Functional Gene Replacement in Drosophila

项目摘要 最近由SARS-CoV-2冠状病毒感染引起的COVID-19大流行， 需要了解这种新疾病发病机制的生物学机制。SARS-CoV-2是一种 一种略小于30 kb长的RNA基因组，编码26种蛋白质， 与数百种人类蛋白质结合并启动许多致病步骤。在这里，我们使用遗传 以果蝇为模型，了解与COVID-19相关的基因和蛋白质功能。我们将 产生UAS-cDNA构建体和转基因果蝇的文库，以表达和功能评估病毒 proteins.我们还将为人类基因组中涉及病毒的300多个基因培育转基因果蝇。 复制和COVID-19疾病。我们将为这些果蝇同源物生产果蝇试剂， 人类基因使用一种独特的插入式技术，我们将更新在线中心， 将这些资源提供给世界各地的果蝇实验室。

PROJECT SUMMARY The recent pandemic of COVID-19 due to infection with the SARS-CoV-2 coronavirus has created an urgent need to understand the biological mechanisms of pathogenesis of this new disease. The SARS-CoV-2 is a coronavirus with a slightly less than 30 kilobases (kb) long RNA genome that encodes 26 proteins that interact with several hundred human proteins and initiate a number of pathogenic steps. Here we use the genetic model Drosophila melanogaster to understand gene and protein function involved in COVID-19. We will generate a library of UAS-cDNA constructs and transgenic flies to express and functionally assess the viral proteins. We will also generate transgenic flies for over 300 genes in the human genome involved in viral replication and COVID-19 disease. We will generate Drosophila reagents for the Drosophila homologs of these human genes using a unique drop-in technology and we will update the online hub for the dissemination of these resources to Drosophila labs throughout the world.