Adipose Tissue Biology and Nutrient Metabolism Core

摘要：脂肪细胞生物学与营养代谢核心 脂肪组织生物学和营养代谢核心的目的是： 1.促进和促进对白色、米色和棕色脂肪组织质量调节机制的研究， 健康和疾病中的分布、营养信号和仓库特有的代谢和内分泌功能。 2.提供简单和经济高效的途径，获得质量严格控制的啮齿动物和人类前脂肪细胞，并 脂肪细胞和储存的细胞和组织及其产物(例如，RNA、cDNA、组织或细胞裂解产物， 条件媒体)。 3.根据本研究基地的需要，发展和规范脂肪细胞和胰岛生物学的方法 快速发展的领域。 4.从以下方面为新调查人员和现有调查人员提供咨询意见、支持和培训 营养和肥胖领域。 5.在新陈代谢的准备和质量评估方面提供咨询意见、支持和培训 组织包括肝细胞、胰岛和人成纤维细胞。 6.促进和促进将细胞和动物模型的基础研究成果转化为 临床/转化性研究。 7.组织脂肪和新陈代谢组织系列研讨会，作为新的 合作，并作为博士后学生和博士后研究员的培训基地。 8.组织关于有争议的主题和新技术在我们领域的应用的研讨会。 核心所提供的服务 1.脂肪组织的采购和加工 2.间质血管细胞的分离和脂肪干细胞的制备 3.ASCs/前体脂肪细胞向脂肪细胞分化： 4.激素和细胞因子测定。组织碎片或细胞分泌脂肪因子 5.啮齿动物脂肪组织的解剖与收获。 6.关于代谢研究最佳方法的标准化方案和分析技术及建议(例如 代谢物、脂肪分解、葡萄糖吸收和代谢、脂肪生成、激素信号) 计划中的新ABM核心服务： ·使用海马氧分析仪进行细胞呼吸和生物能量学研究。 ·从啮齿动物肝脏和脂肪细胞中分离线粒体 ·胶原酶消化法分离大鼠胰岛

ABSTRACT: ADIPOCYTE BIOLOGY and NUTRIENT METABOLISM CORE The purposes of the Adipose Tissue Biology and Nutrient Metabolism Core are to: 1. Facilitate and foster research on mechanisms regulating white, beige and brown adipose tissue mass, distribution, nutrient signaling and depot-specific metabolic and endocrine functions in health and disease. 2. Provide easy and cost-effective access to carefully quality controlled rodent and human preadipocytes and adipocytes, and banked cell and tissues and their products (e.g. RNA, cDNA, tissue or cell lysates, conditioned media). 3. Develop and standardize methods in adipocyte and islet biology as needed by our research base in this rapidly evolving field. 4. Provide consultative advice, support and training for new investigators and established investigators from the nutrition and obesity fields. 5. Provide consultative advice, support and training in the preparation and quality assessment of metabolic tissues including hepatocytes, islets and human fibroblasts. 6. Facilitate and foster the translation of basic research findings from cell and animal models into clinical/translational studies. 7. Organize the Adipose and Metabolic Tissue Seminar Series, which serves as an incubator for new collaborations, and as a training ground for pre-doctoral students and post-doctoral fellows. 8. Organize workshops on controversial topics and the application of new technologies to our field. Services Provided By The Core 1. Adipose tissue procurement and processing 2. Isolation of stromal vascular cells and preparation of adipose stem cells (ASCs) 3. Differentiation of ASCs/preadipocytes into adipocytes: 4. Hormone and cytokine assays. Secretion of adipokines from tissue fragments or cells 5. Dissection and harvest of rodent adipose tissues. 6. Standardized protocols and analytical techniques and advice on optimal methods for metabolic studies (e.g. metabolites, lipolysis, glucose uptake and metabolism, lipogenesis, hormone signaling) Planned New ABM Core Services: • Cell respiration and bioenergetics with the Seahorse Oxygen Analyzer. • Mitochondria isolation from rodent liver and fat cells • Islet isolation from rats or mice by collagenase digestion