Live Cell Imaging Shared Resource

西蒙斯综合癌症中心(SCCC)的活细胞成像共享资源(LCISR)使 SCCC成员利用先进的光学显微镜技术支持他们专注于癌症的研究。 LCISR的能力包括组织学染色样本的数字成像，主要的共聚焦 显微镜，用于活体动物和厚组织成像的精密双光子显微镜 反射荧光(TIRF)显微镜和多种模式的超分辨率成像，以及两种 配备全面图像分析软件的图像处理工作站。在凯特的领导下 卢比-菲尔普斯，博士，LCISR工作人员为用户提供个性化培训，并就实验提供咨询 设计和图像分析。在评估新的仪器设备和获得 为购买仪器设备提供资金。自2015年以来，LCISR更换了两个老化的激光扫描共焦 在NIH S10计划的资助下，该公司还增加了第三台共焦显微镜，从而扩大了其能力。 这项收购包括一台蔡司AiryScan快速附件，用于“超分辨率”成像和一台荧光灯 用于研究溶液和活细胞中分子相互作用的关联光谱模块是独一无二的 在德克萨斯大学西南医学中心(UTSW)校园里。LCISR最近收购了一台OMX SR多模 具有3D结构照明显微镜、定位显微镜的超分辨率显微镜 (Palm/Storm)和Ring TIRF功能。为了跟上光学显微镜的持续快速发展 在技术方面，LC ISR最近启动了与UTSW显微镜创新实验室(MIL)的合作伙伴关系， 由凯文·迪恩博士领导。MIL是一个新建立的、得到制度支持的倡议，旨在快速通道 将尖端光学显微镜成像仪器翻译给UTSW的研究人员。计划中的 仪器包括定制的光片显微镜，可进行清晰的组织成像和快速体积测量 成像，以及用于深层组织成像的三光子显微镜。由MIL开发的仪器将制造 可通过LC ISR向SCCC成员提供。自2014年以来，已有140多人使用了LCISR 所有五个SCCC研究项目的主要研究人员，导致140多个专注于癌症的项目 出版物，包括在《细胞》、《自然》、《科学》、《癌细胞》、《自然》等影响较大的期刊上发表的出版物 通讯、发展细胞和自然微生物学。每年使用SCCC成员实验室的数量 LCISR已从2015年的54项扩大到2019年的80项。

The Live Cell Imaging Shared Resource (LCISR) of Simmons Comprehensive Cancer Center (SCCC) enables SCCC members to leverage advanced light microscopy techniques in support of their cancer-focused research. The LCISR’s capabilities include digital imaging of histologically stained samples, workhorse confocal microscopes, sophisticated two-photon microscopes for imaging in live animals and thick tissues, total internal reflection fluorescence (TIRF) microscopes, and multiple modes of super-resolution imaging, as well as two image processing workstations with comprehensive image analysis software. Under the leadership of Kate Luby-Phelps, PhD, LCISR staff members provide individualized training of users and consult on experimental design and image analysis. The LCISR has been proactive in evaluating new instrumentation and in obtaining funding for instrumentation purchase. Since 2015, the LCISR has replaced two aging laser scanning confocal microscopes and has expanded its capacity by adding a third confocal with funding from the NIH S10 program. This acquisition included a Zeiss Airyscan FAST attachment for “super resolution” imaging and a fluorescence correlation spectroscopy module for examining molecular interactions in solution and in living cells that is unique on the UT Southwestern Medical Center (UTSW) campus. The LCISR recently acquired an OMX SR multimode super-resolution microscope with 3D structured illumination microscopy, localization microscopy (PALM/STORM), and Ring TIRF capabilities. To keep up with ongoing rapid developments in light microscope technology, the LCISR recently initiated a partnership with the UTSW Microscope Innovation Laboratory (MIL), led by Kevin Dean, PhD. The MIL is a newly established, institutionally supported initiative intended to fast-track the translation of cutting-edge light microscope imaging instrumentation to researchers at UTSW. Planned instrumentation includes custom-built lightsheet microscopes for cleared tissue imaging and fast volumetric imaging, and a three-photon microscope for deep tissue imaging. Instruments developed by the MIL will be made available to SCCC members through the LCISR. Since 2014, the LCISR has been used by more than 140 principal investigators across all five SCCC research programs, resulting in more than 140 cancer-focused publications, including publications in high-impact journals such as Cell, Nature, Science, Cancer Cell, Nature Communications, Dev Cell, and Nature Microbiology. The annual number of SCCC member laboratories using the LCISR has expanded from 54 in 2015 to 80 in 2019.