Live Cell Imaging Shared Resource

哈罗德·西蒙斯癌症中心 活细胞成像共享资源(翻译研究资源) 项目摘要/摘要 活细胞成像共享资源维护并提供对10台光学显微镜和3台光学显微镜的访问 图像处理工作站。显微镜的范围从相对简单的拍照仪器到 从组织学染色的样品到精密的双光子显微镜，用于活体动物和厚壁动物的成像 纸巾。训练有素、值得信赖的用户可以按小时付费使用显微镜。设施工作人员提供个人 培训和援助。该资源是服务于整个德克萨斯大学西南部的机构核心设施 研究社区。癌症中心会员占设施用户的50%以上，70% 乐器每小时的使用情况。资源总监和设施经理提供个人培训 免费为癌症中心的使用者提供帮助，同时向会员收取服务费 根据癌症中心提供的支持金额减少。 服务还包括关于协议开发的建议、实验故障排除和帮助 对成像数据进行适当的解释和有效的呈现。希望使用该设施的调查人员 通常与总监会面，讨论哪种显微镜最适合他们的项目，并 确定合适的样品制备和实验设计方案。署长担任 需要常规显微镜成像以外的项目的顾问或合作者。设施经理 培训新用户。为了最大限度地提高培训的效率和效果，用户会根据实际样本进行培训 他们将被想象，并且只被教授与他们的实验有关的特征。设施经理是 可在正常工作时间内为用户提供帮助。经批准的用户可以在下班后借出密钥 进入。一个基于网络的日历系统被用来预留时间。它与一个用户帐户集成在一起 数据库和用于跟踪实际使用情况的身份验证服务器。

Harold C. Simmons Cancer Center Live Cell Imaging Shared Resource (Translational Research Resource) Project Summary/Abstract The Live Cell Imaging Shared Resource maintains and provides access to ten light microscopes and three image processing workstations. The microscopes range from relatively simple instruments for taking pictures of histologically stained samples to sophisticated two photon microscopes for imaging in live animals and thick tissues. Trained, trusted users may use the microscopes for an hourly fee. Facility staff provides individual training and assistance. The resource is an institutional core facility serving the whole UT Southwestern research community. Cancer Center members account for more than 50% of the users of the facility and 70% of the hourly use of the instruments. The Resource Director and Facility Manager provide individual training and assistance to Cancer Center users of the facility at no cost, while fees charged to members for services are reduced according to the amount of support provided by Cancer Center. Services also include advice on protocol development, troubleshooting of experiments and help with the appropriate interpretation and effective presentation of imaging data. Investigators who wish to use the facility typically meet with the Director to discuss which microscope would be most suitable for their project and to determine appropriate protocols for sample preparation and experimental design. The Director serves as consultant or collaborator on projects requiring more than routine microscope imaging. The facility manager trains new users. To maximize the efficiency and efficacy of training, users are trained on the actual specimens they will be imaging and are only taught the features that pertain to their experiment. The facility manager is available during normal working hours to assist users. Approved users may check out a key for after-hours access. A web-based calendaring system is used to reserve time. It is integrated with a user accounts database and an authentication server for tracking actual usage.