Animal Models Core

项目摘要/摘要 转基因小鼠的使用极大地扩展了我们对肾脏发育、遗传学、 生理学和病理生理学。德克萨斯大学西南部奥布莱恩中心动物模型核心(核心A) 为社区服务了10年，并将继续为希望 为研究肾功能和疾病建立小鼠模型。核心将服务于多种目的。 我们为通常不研究肾脏的小鼠遗传学家提供必要的试剂和专业知识。 以促进和鼓励他们进入这一领域。我们鼓励不经常使用鼠标的调查人员 模型，包括特别是临床研究人员，冒险进入老鼠系统并缓解这种情况 过渡。我们还将协助建立急性和慢性肾脏疾病的非遗传模型 研究人员在他们的研究中。我们将通过几种方式实现我们的目标。首先，核心已经产生了 并将分布许多肾脏特异的条件性(可诱导激活物和抑制物)、肾小球或肾小管 片段特异的Cre重组酶品系将与感兴趣的开花或tet响应品系杂交 调查员。我们将提供建议以及实际的老鼠线。第二，核心人员将协助 根据合作研究人员的要求，产生额外的转基因小鼠、小鼠和细胞系中的基因敲除。我们会 要么提供建议，要么如果需求很高，我们计划每年在我们的核心增加一条新的生产线。最后，核心 工作人员将执行或培训调查人员使用各种器官损伤/修复模式以及 活体培养。我们将始终拥有探索模块，在那里我们将参与尖端技术 在肾脏研究方面。我们已经开发了肾脏的4-D实时成像、代谢物标记和 器官培养的量化，器官培养的代谢组学，CRISPR-Cas9介导的基因组工程， 肾单位祖细胞的分离、扩增和分化。这个核心的最终目的是 促进肾脏发育、遗传学、生理学和病理生理学的研究。

PROJECT SUMMARY/ABSTRACT The use of genetically modified mice has greatly expanded our understanding of kidney development, genetics, physiology, and pathophysiology. The UT Southwestern O'Brien Center Animal Models Core (Core A) has served the community for 10 years and will continue to provide cutting edge technology to investigators wishing to generate mouse models for the study of kidney function and disease. The core will serve multiple purposes. We provide the necessary reagents and expertise to mouse geneticists who do not normally work with the kidney to facilitate and encourage their move into this field. We encourage investigator who do not normally use mouse models, including and especially clinical investigators, to venture into the mouse system and to ease this transition. We will also assist the generation of non-genetic models of acute and chronic kidney disease to assist investigators in their studies. We will accomplish our objectives in several ways. First, the core has generated and will distribute many kidney-specific conditional (inducible activator and repressor lines), glomerular or tubule segment-specific Cre recombinase lines to be crossed to floxed or tet responder lines of interest to the investigator. We will provide advice as well as the actual mousselines. Second, core personnel will assist in the generation of additional transgenic mice, knockout/in mice and cell lines as required by co-investigators. We will either offer advice or if the demand is high, we plan to add a new line t our Core once a year. Finally, core personnel will perform for or train investigators in the use of various models of organ injury/repair as well as ex vivo culture. We will always have exploratory modules where we participate in the cutting edge of technologies in renal research. We have developed technologies for 4-D live imaging of kidneys, metabolite labeling and quantification in organ culture, metabolomics in organ culture, CRISPR-Cas9 mediated genome engineering, isolation, expansion and differentiation of nephron progenitor cells. The ultimate purpose of this core is to facilitate research on kidney development, genetics, physiology and pathophysiology.