Community Crystallization Resource for Biological Macromolecules

1R24GM12135生物大分子社区结晶资源 行政补充支持请求 项目摘要/摘要 大分子X射线结晶学是用于结构分析的主要方法之一 生物学，占蛋白质数据库中已解决结构的~90%，蛋白质数据库是主要的存储库 和公共数据库，用于使这些生物分子的三维结构可供 各个领域的研究人员。要做结晶学，我们需要晶体。查找符合以下条件的条件 在这一过程中，大分子的结晶仍然是一个关键的挑战，我们关注的是 在高通量结晶筛选中心解决这一问题。结晶化 中心是一个社区资源，提供结晶实验设置和成像 独特设计的1,536种结晶条件。关于大分子的信息 高效地收集结晶，从而使整个研究的结构调查 社区可以快速发展。结晶中心拥有强大的成像时间表， 有助于立即确定积极的结晶结果。不可预见的事情 新冠肺炎疫情蔓延造成的情况扰乱了 结晶中心，以及宽基结晶的研究活动 中心用户。此修订申请寻求资金来解决这一意外的维护需求 在结晶中心进行操作，以便于提供连续不间断 服务。

1R24GM12135 COMMUNITY CRYSTALLIZATION RESOURCE FOR BIOLOGICAL MACROMOLECULES ADMINISTRATIVE SUPPLEMENT SUPPORT REQUEST PROJECT SUMMARY/ABSTRACT Macromolecular X-ray crystallography is one of the predominant methods used in structural biology, accounting for ~90% of solved structures in the Protein Data Bank, the primary repository and public database for making these three-dimensional structures of biomolecules available to researchers in all fields. To do crystallography, we need crystals. Finding conditions in which a macromolecule will crystallize remains a key challenge in this process, and we have focused on solving this problem at the High-Throughput Crystallization Screening Center. The Crystallization Center is a community resource that provides crystallization experiment set-up and imaging of a uniquely designed set of 1,536 crystallization conditions. Information about macromolecular crystallization is efficiently collected, so that structural investigations for the entire research community can rapidly progress. The Crystallization Center has a robust imaging schedule that facilitates immediate identification of positive crystallization outcomes. The unforeseen circumstances arising due to the extended COVID-19 pandemic have disrupted the operations of the Crystallization Center, as well as the research activities of the wide base of Crystallization Center users. This revision application seeks funds to address this unanticipated need to maintain operations in the Crystallization Center and to facilitate providing continuous uninterrupted services.