CryoProbe-enabled Bruker AVANCE NEO 500 MHz NMR Spectrometer

项目总结/摘要 磁共振中心（MRC）是一个集中的设施，拥有唯一的高- 现场核磁共振波谱仪在波士顿学院（BC）。MRC主要服务于教师 化学系的成员，其中67%的人在由资助的项目工作 国家卫生研究院液态核磁共振是迄今为止最适合于生物医学的实验方法 研究，因为正在调查的样品最接近模仿分子在 它们在我们体内的自然状态在此，要求提供资金， 启用来自Bruker Biospin Corporation的AVANCE NEO 500 MHz光谱仪。这 系统可以以最高的灵敏度检测非常低（nM）浓度的样品， 同时减少数据收集的采集时间。目前的核磁共振系统 占领MRC是由安捷伦制造的，安捷伦已经停止了在 因此，对现有文书的支持将于2021年结束。的 500 MHz近地天体具有更高的灵敏度和分辨率，将取代400 MHz系统， 一块有二十多年历史的磁铁。除了满足这一关键需求外， CryoProbe将提高NMR数据的灵敏度，同时增加MRC的容量 由于减少了采集时间。这是一个配件，从来没有一部分的BC的 乐器曲目。新系统亦有助管理局维持其 BC的职能是为我们的研究人员提供目前正在开发的新功能 不可用. BC的许多NIH资助的研究项目强调 改善公共卫生，重点是生物系统或新的催化方法 帮助药物设计一个足够灵敏的核磁共振光谱仪， 材料较少的高质量数据对于促进这些项目的成果至关重要。的 新仪器的能力和灵敏度的提高将使所有这些NIH受益 BC的项目，其中包括生物途径的研究，以及新的 生物治疗学新系统也非常擅长氘检测， 在低温下容易获得光谱，并具有水抑制技术 比现有的安捷伦同类产品更上级、更用户友好。所有这些 这些方面对于支持BC正在进行的项目至关重要，这些项目在历史上一直受到限制 由传统的安捷伦系统。因此，这一新工具将被证明是一个 我们的研究人员在未来几年的宝贵资产。

Project Summary/Abstract The Magnetic Resonance Center (MRC) is a centralized facility housing the only high- field NMR spectrometers at Boston College (BC). The MRC primarily serves the faculty members of the Chemistry Department, 67% of whom work on projects funded by the NIH. Liquid state NMR is by far the most suitable experimental method for biomedical research, because the samples under investigation most closely mimic the molecules in their natural state within our bodies. Here, funds are requested to purchase a CryoProbe enabled AVANCE NEO 500 MHz spectrometer from Bruker Biospin Corporation. This system can detect samples of very low (nM) concentrations with the highest sensitivity, while reducing the acquisition time for data collection. The NMR systems that currently occupy the MRC were manufactured by Agilent, which has ceased all operations in magnetic resonance; therefore, support for the existing instruments will end in 2021. The 500 MHz NEO, with higher sensitivity and resolution, will replace a 400 MHz system with a magnet that is over twenty years old. In addition to addressing this critical need, the CryoProbe will enhance sensitivity of the NMR data while increasing capacity in the MRC due to reduced acquisition times. This is an accessory that has never been a part of BC’s instrumentation repertoire. The new system will also enable the MRC to maintain its vital function at BC by providing our researchers with new capabilities that are currently unavailable. The numerous NIH-funded research projects at BC emphasize on the betterment of public health, focusing on biological systems or novel catalysis methods that aid in drug design. An NMR spectrometer that is sensitive enough to acquire high quality data with less material is crucial for promoting the outcomes of these projects. The enhanced capacity and sensitivity of the new instrument will benefit all of these NIH projects at BC, which include studies of biological pathways, and developments of new biologic therapeutics. The new system is also very adept at deuterium detection, enables facile acquisition of spectra at low temperatures, and has water suppression techniques that are superior and more user-friendly than existing Agilent counterparts. All of these aspects are crucial to support ongoing projects at BC that have historically been limited by the legacy Agilent systems. Therefore, this new instrument will prove to be an invaluable asset to our researchers for years to come.